Neudesin Antibody - BSA Free

Images

 
Western Blot: Neudesin Antibody [NBP1-77331] - Human kidney tissue lysate with NENF antibody at (A) 1 and (B) 2 ug/mL.
Western Blot: Rabbit Polyclonal Neudesin Antibody [NBP1-77331] - Analysis of mouse liver tissue lysates diluted with 1X TBST at concentration of 1 ug/ml. Image from a verified customer review.
Immunocytochemistry/ Immunofluorescence: Neudesin Antibody - BSA Free [NBP1-77331] - Immunofluorescence of Neudesin in rat kidney tissue with Neudesin antibody at 20 ug/mL.
Immunohistochemistry: Neudesin Antibody - BSA Free [NBP1-77331] - Immunohistochemistry of Neudesin in rat kidney tissue with Neudesin antibody at 10 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Neudesin Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human NENF. The immunogen is located within the last 50 amino acids of NENF. Amino Acid Squence: DGSPNLDFKPEDQPH
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NENF
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Despite its predicted molecular weight of ~20kDa, Neudesin is observed at 37 kDa in SDS-PAGE.
Control Peptide
Neudesin Peptide (NBP1-77331PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Neudesin Antibody - BSA Free

  • Cell immortalization-related protein 2
  • CIR2
  • CIR2cell growth-inhibiting protein 47
  • NENF
  • Neudesin
  • neuron derived neurotrophic factor
  • Neuron-derived neurotrophic factor
  • SCIRP10
  • SCIRP10-related protein
  • Secreted protein of unknown function
  • Spinal cord injury related protein 10
  • SPUF protein
  • SPUF
  • SPUFneudesin

Background

NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37kDa in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NENF
Uniprot