Neudesin Antibody - BSA Free

Western Blot: Neudesin Antibody [NBP1-77331] - Human kidney tissue lysate with NENF antibody at (A) 1 and (B) 2 ug/mL.

Western Blot: Rabbit Polyclonal Neudesin Antibody [NBP1-77331] - Analysis of mouse liver tissue lysates diluted with 1X TBST at concentration of 1 ug/ml. Image from a verified customer review.

Immunocytochemistry/ Immunofluorescence: Neudesin Antibody - BSA Free [NBP1-77331] - Immunofluorescence of Neudesin in rat kidney tissue with Neudesin antibody at 20 ug/mL.

Immunohistochemistry: Neudesin Antibody - BSA Free [NBP1-77331] - Immunohistochemistry of Neudesin in rat kidney tissue with Neudesin antibody at 10 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Neudesin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Neudesin Antibody - BSA Free (NBP1-77331) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human NENF. The immunogen is located within the last 50 amino acids of NENF. Amino Acid Squence: DGSPNLDFKPEDQPH
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NENF
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Despite its predicted molecular weight of ~20kDa, Neudesin is observed at 37 kDa in SDS-PAGE.
• Control Peptide:

  Neudesin Peptide (NBP1-77331PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Neudesin Antibody - BSA Free

• Cell immortalization-related protein 2
• CIR2
• CIR2cell growth-inhibiting protein 47
• NENF
• Neudesin
• neuron derived neurotrophic factor
• Neuron-derived neurotrophic factor
• SCIRP10
• SCIRP10-related protein
• Secreted protein of unknown function
• Spinal cord injury related protein 10
• SPUF protein
• SPUF
• SPUFneudesin

Background

NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37kDa in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NENF
• Uniprot:
  • NP_037481(Human)