NEU2 Antibody

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Western Blot: NEU2 Antibody [NBP2-21649] - Affinity Purified anti-Neu2 antibody to detect recombinant His tagged Neu-2. Molecular weight marker indicates a single band of the expected MW. The blot was incubated with a ...read more
Western Blot: NEU2 Antibody [NBP2-21649] - Immunochemicals Affinity Purified anti-Neu2 antibody to detect Neu-2 present in a lysate expressing human Neu2. Molecular weight marker indicates a band of the expected MW. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NEU2 Antibody Summary

Immunogen
The synthetic peptide corresponding to amino acids range from 110-133 of Human Neu2. Protein accession number 222352169. The target domain of reactivity is Internal.
Specificity
This antibody reacts with human Neu2. Cross-reactivity against Neu1 has not yet been established.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NEU2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000-1:50000
Application Notes
This antibody is suitable for western blotting, immunocytochemistry, immunoprecipitation, transfected cell culture, primary cell culture, and ELISA. A single band of the expected apparent molecular weight (43 kDa) was observed at a 1:500 dilution incubated for 1 h at room temperature. A second lower molecular weight band may represent a truncated form of this protein. Neuraminidase is not very abundant in most tissues and its detection using this antibody may require further optimization. Researchers should determine optimal titers for other applications.

Reactivity Notes

Reactive in Human. Based on sequence we expect this antibody to react with neuraminidase from other sources, although specific reactivity has not been confirmed. Neuraminidases are highly conserved in mammals and therefore cross reactivity is expected with mouse and rat Neu2.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NEU2 Antibody

  • Cytosolic sialidase
  • EC 3.2.1.18
  • N-acetyl-alpha-neuraminidase 2
  • neuraminidase 2
  • SIAL2MGC129579
  • sialidase 2 (cytosolic sialidase)
  • sialidase-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NEU2
Uniprot