Nephronectin Antibody [Alexa Fluor™ Plus 488]

• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 488

Nephronectin Antibody [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects mouse Nephronectin in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nephronectin Antibody [Alexa Fluor™ Plus 488]

• EGFL6L
• Nctn
• Nephronectin
• NPNT
• POEM

Background

Nephronectin, also named POEM (preosteoblast EGF repeat protein with MAM domain) is a 70‑90 kDa extracellular matrix protein that is a ligand for integrin alpha ₈ beta ₁ (1, 2). The 561 amino acid (aa) mouse Nephronectin (isoform b) contains a 19 aa signal sequence, a matrilin-type vWA domain, two calcium-binding EGF-like domains, a potential N-linked glycosylation site, a pro/ser/thr-rich mucin-like region, an RGD integrin binding motif, and a MAM domain. These features are often present in matrix proteins that, like Nephronectin, mediate cell adhesion and spreading (1, 2). Isoform a includes a 17 aa insertion between aa 58 and 59 that is missing in isoform b. Mouse Nephronectin shows 88%, 92%, 85% and 77% aa identity with human, rat, bovine and opossum Nephronectin, respectively. It is most highly expressed in developing endocrine organs such as parathyroid, thyroid, hypophysis and pineal organ, and around developing bone, teeth, and muscle (1, 2). Pre-osteoblast cells produce Nephronectin, but down‑regulate it as they differentiate (1). It is also expressed in the Wolffian duct and ureteric bud basement membranes in the developing kidney, where it is colocalizes, and forms an in-vivo complex with integrin alpha ₈ beta ₁ (2). Since deletion of integrin alpha ₈ beta ₁ results in renal agenesis, Nephronectin has been proposed to be a critical integrin alpha ₈ beta ₁ ligand during Nephrogenesis (2, 3).

1. Morimura, N. et al. (2001) J. Biol. Chem. 276:42172.
2. Brandenberger, R. et al. (2001) J. Cell Biol. 154:447.
3. Miner, J. H. (2001) J. Cell Biol. 154:257.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.