Nephronectin Antibody (859326) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 859326
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Nephronectin Antibody (859326) [Alexa Fluor® 647] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Nephronectin
  Glu20-Arg565
  Accession # Q6UXI9
• Specificity: Detects human Nephronectin in ELISAs and Western blots.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nephronectin Antibody (859326) [Alexa Fluor® 647]

• EGFL6L
• Nctn
• Nephronectin
• NPNT
• POEM

Background

Nephronectin, also called POEM (preosteoblast EGF repeat protein with MAM domain) or EGFL6L, is a 70‑90 kDa extracellular matrix protein that is a ligand for integrin alpha 8 beta 1 and mediates cell adhesion and spreading. The 565 amino acid (aa) human Nephronectin contains a 19 aa signal sequence, five EGF-like domains, a mucin-like region, an RGD integrin binding motif, and a MAM domain. Isoforms of 509-595 aa contain deletions of aa 387-416, and/or an insertion of 30 aa after aa 88, and/or substitution of 3 aa for aa 535-564. Mature human Nephronectin shares 88% and 89% aa sequence identity with mouse and rat Nephronectin, respectively. It is most highly expressed in developing endocrine organs such as parathyroid, thyroid, hypophysis and pineal organ, around developing bone, teeth, and muscle, and in the Wolffian duct and ureteric bud basement membranes in the developing kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.