NEK9 Antibody

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Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NEK9 Antibody Summary

Immunogen
Synthetic peptide: C-KEVDLTRLSEKERRD, representing the internal region of the human protein according to NP_149107.3
Clonality
Polyclonal
Host
Goat
Gene
NEK9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA 1:16000
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 75-80kDa band in Human Heart and Testes lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kDa according to NP_149107.3. The 75-80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Positive Control
NEK9 Lysate (NBL1-13588)

Reactivity Notes

Human, mouse, rat and dog.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for NEK9 Antibody

  • DKFZp434D0935
  • KIAA1995
  • MGC138306
  • MGC16714
  • NEK8
  • Nek8NERCC1
  • Nercc1 kinase
  • NERCCEC 2.7.11.1
  • Never in mitosis A-related kinase 9
  • NIMA (never in mitosis gene a)- related kinase 9
  • NimA-related kinase 8
  • NIMA-related kinase Nek8
  • NimA-related protein kinase 9
  • serine/threonine-protein kinase Nek9

Background

NEK9 is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. It phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. NEK9 phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NEK9
Entrez
Uniprot