NEDD1 Antibody (7D10) Summary
Immunogen
NEDD1 (NP_690869, 561 a.a. ~ 660 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AGVASSLSEKIADSIGNNRQNAPLTSIQIRFIQNMIQETLDDFREACHRDIVNLQVEMIKQFHMQLNEMHSLLERYSVNEGLVAEIERLREENKRLRAHF
Marker
Centrosome Marker
Specificity
NEDD1 - neural precursor cell expressed, developmentally down-regulated 1
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NEDD1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Applications/Dilutions
Dilutions
ELISA 1:100-1:2000 Immunoblotting Immunocytochemistry/Immunofluorescence Immunohistochemistry 1:10-1:500 Immunohistochemistry-Paraffin 1:10-1:500 Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24025740).
Publications
Read Publications using H00121441-M05 in the following applications:
Reactivity Notes
Other species have not been tested.
Packaging, Storage & Formulations
Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for NEDD1 Antibody (7D10)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Applications: Flow, ICC/IF, IHC, IHC-P, WB
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Applications: ICC/IF, IHC, IHC-P, WB
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Applications: Flow, IHC, IHC-P, WB
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Applications: ICC/IF, IHC, IHC-P, IP, WB
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Publications for NEDD1 Antibody (H00121441-M05)(11)
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 2 applications: ICC/IF, WB.
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Showing Publications 1 -
10 of 11.
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Publications using H00121441-M05
Applications
Species
Baumann C, Wang X, Yang L, Viveiros MM. Error-prone meiotic division and subfertility in mice with oocyte-conditional knockdown of pericentrin. J. Cell. Sci. 2017 Feb 13 [PMID: 28193732] (Mouse)
Mouse
Uehara R, Kamasaki T, Hiruma S et al. Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission. Mol Biol Cell 2016 Mar 1 [PMID: 26764096] (WB)
WB
Lecland N, Luders J. The dynamics of microtubule minus ends in the human mitotic spindle. Nat Cell Biol. 2014 Jun 29 [PMID: 24976384]
Tamura AN, Huang TT, Marikawa Y. Impact of Vitrification on the Meiotic Spindle and Components of the Microtubule-Organizing Center in Mouse Mature Oocytes. Biol Reprod. 2013 Sep 11 [PMID: 24025740] (ICC/IF, Mouse)
ICC/IF
Mouse
Uehara R, Goshima G. Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase. Uehara R, Goshima G. J Cell Biol. [PMID: 20937700]
Archinti M, Lacasa C, Teixido-Travesa N, Luders J. SPICE--a previously uncharacterized protein required for centriole duplication and mitotic chromosome congression. J Cell Sci 123(Pt 18):3039-46. 2010 Sep 15. [PMID: 20736305]
Stiess M, Maghelli N, Kapitein LC et al. Axon extension occurs independently of centrosomal microtubule nucleation. Science 327(5966):704-7. 2010 Feb 5. [PMID: 20056854]
Haren L, Stearns T, Luders J. Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components. PLoS One. 2009 Jun 19 [PMID: 19543530]
Zhu H, Coppinger JA, Jang CY et al. FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle. J Cell Biol 183(5):835-48. 2008 Dec 1. [PMID: 19029337]
Teixido-Travesa N, Villen J, Lacasa C et al. The {gamma}TuRC Revisited: A Comparative Analysis of Interphase and Mitotic Human {gamma}TuRC Redefines the Set of Core Components and Identifies the Novel Subunit GCP8. MBoC Sep 13 2010
Zhu H, Fang K, Fang G. FAM29A, a target of Plk1 regulation, controls the partitioning of NEDD1 between the mitotic spindle the centrosomes. J Cell Sci. 2009 Jul 13. [PMID: 19596795]
Show All 11 Publications.
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