NEIL1 Antibody

Product Discontinued

NEIL1 Antibody Summary

• Immunogen: Synthetic peptide, CKVKADIPSLEPE, representing the C Terminus of the human protein according to NP_078884.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NEIL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA 1:100-1:2000
• Application Notes: Peptide ELISA In Western Blot preliminary experiments gave an approx 75kDa band in Human Liver lysates after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.7kDa according to NP_078884.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
• Control:

  NEIL1 Overexpression Lysate

Reactivity Notes

Expected to react with human due to sequence identity.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEIL1 Antibody

• DNA-(apurinic or apyrimidinic site) lyase Neil1
• EC 3.2.2.-
• EC 4.2.99.18
• endonuclease 8-like 1
• endonuclease VIII
• Endonuclease VIII-like 1
• FLJ22402
• FPG1DNA glycosylase/AP lyase Neil1
• hFPG1
• NEH1
• nei endonuclease VIII-like 1 (E. coli)
• Nei homolog 1
• NEI1
• Nei-like protein 1

Background

NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEIL1
• Entrez:
  • 79661(Human)
• Uniprot:
  • NP_078884.1(Human)