NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody (2E9-D12)

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Western Blot: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody (2E9-D12) [H00008883-M02] - Analysis of NAE1 expression in transfected 293T cell line by NAE1 monoclonal antibody (M02), clone 2E9-D12.Lane 1: NAE1 ...read more
Sandwich ELISA: NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody (2E9-D12) [H00008883-M02] - Detection limit for recombinant GST tagged NAE1 is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2E9-D12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody (2E9-D12) Summary

Immunogen
NAE1 (AAH00480, 1 a.a. - 534 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NAE1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
It has been used for ELISA and WB.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NEDD8 Activating Enzyme (APPBP1/UBA3) Antibody (2E9-D12)

  • A-116A10.1
  • amyloid beta precursor protein binding protein 1
  • amyloid beta precursor protein binding protein 1, 59kDa
  • Amyloid beta precursor protein-binding protein 1, 59 kDa
  • amyloid beta precursor protein-binding protein 1, 59kD
  • Amyloid protein-binding protein 1
  • APPBP1APP-BP1
  • NEDD8 Activating Enzyme (APPBP1/UBA3)
  • NEDD8 activating enzyme E1 subunit 1
  • NEDD8-activating enzyme E1 regulatory subunit
  • NEDD8-activating enzyme E1 subunit
  • protooncogene protein 1
  • Proto-oncogene protein 1
  • ula-1

Background

The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NAE1
Entrez
Uniprot