Nectin-2/CD112 Antibody (502-57)

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, IHC, IP
• Clone: 502-57
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Nectin-2/CD112 Antibody (502-57) Summary

• Immunogen: Raised against the extracellular domain of mouse Nectin 2.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: NECTIN2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: For flow cytometry, Western blotting and immunohistochemistry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Fixation with PFA or acetone/methanol is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Nectin-2/CD112 Antibody (502-57)

• CD112 antigen
• CD112
• Herpes virus entry mediator B
• Herpesvirus entry mediator B
• herpesvirus entry protein B
• HVEB
• HVEBpoliovirus receptor-like 2
• MPH
• nectin 2
• Nectin2
• Nectin-2
• poliovirus receptor-related 2 (herpesvirus entry mediator B)
• poliovirus receptor-related protein 2
• PRR2
• PRR2nectin-2
• PVRL2
• PVRR2poliovirus receptor related 2

Background

Nectin (originally isolated as poliovirus receptor-related protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin family consist of nectin-1, nectin-2 and nectin-3. Nectin-2 also known as PRR2 or CD112 is a plasma membrane adhesion molecule localized at adherens junctions which is widely expressed in various cell lines including neuronal, endothelial, epithelial and hematopoietic cells. Next to its role in adherens junctions it functions as alphaherpus virus receptor and acts also as intercellular adhesion molecule and pseudorabies virus receptor. Disruption of mouse nectin-2 leads to infertility of male mice. Monoclonal antibody 502-57 has been raised against the extracellular domain of mouse nectin-2. The antibody cross reacts with human nectin-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NECTIN2