NECAB1 Antibody (CL0580) - BSA Free

Analysis in human cerebral cortex and pancreas tissues using NBP2-52944 antibody. Corresponding NECAB1 RNA-seq data are presented for the same tissues.

Western Blot: NECAB1 Antibody (CL0580) [NBP2-52944] - Lane 1: Marker [kDa], Lane 2: Human cerebral cortex lysate

Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Staining of human cerebellum shows moderate cytoplasmic positivity in neurons.

Staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL0580
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

NECAB1 Antibody (CL0580) - BSA Free Summary

• Description: Novus Biologicals Mouse NECAB1 Antibody (CL0580) - BSA Free (NBP2-52944) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q8N987, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NECAB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  NECAB1 Recombinant Protein Antigen (NBP2-52944PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for NECAB1 Antibody (CL0580) - BSA Free

• EF hand calcium binding protein 1
• EFCBP1
• EF-hand calcium binding protein 1
• EF-hand calcium-binding protein 1
• neuronal calcium binding protein
• Neuronal calcium-binding protein 1
• N-terminal EF-hand calcium binding protein 1
• N-terminal EF-hand calcium-binding protein 1
• STIP-1
• synaptotagmin interacting protein 1

Background

The NECAB1 gene codes for a N-terminal EF-hand calcium-binding protein 1 that in isoform 1 is 351 amino acids long and 40 kDA and in isoform 2 is 100 amino acids long at 11 kDA. NECAB1 has been linked to diseases such as seizures, schizophrenia, alzheimer's disease, motor neuron disease, neuronitis, and neurodegenerative disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NECAB1