NEBL Antibody

• Reactivity: Hu, Mu, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NEBL Antibody Summary

• Immunogen: Peptide with sequence C-ELQRLKRTQE corresponding to internal region according to NP_998734.1, NP_001166955.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize isoform 2 and isoform 3 (NP_998734.1; NP_001166955.1) only, but does not recognize isoform 1 (NP_006384.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NEBL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:4000
• Application Notes: WB: Preliminary experiments gave an approx. 30 kDa band in human brain (frontal cortex) lysates, but not in human, mouse and rat heart lysates after 1 ug/ml antibody staining. This protein has a calculated MW of 31.2 kDa band according to NP_998734.1. The 30 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P usage is reported in scientific literature (PMID: 24449343).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NEBL Antibody

• Actin-binding Z-disk protein
• FLJ53769
• LASP2
• LIM-nebulette
• LNEBL
• MGC119746
• MGC119747
• nebulette

Background

NEBL binds to actin and plays an important role in the assembly of the Z-disk

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NEBL Antibody (NBP1-45223)(4)

Publications using NBP1-45223

• Vejandla RM, Orgil BO, Alberson NR et al. Deficiency in Nebulin Repeats of Sarcomeric Nebulette is Detrimental for Cardiomyocyte Tolerance to Exercise and Biomechanical Stress American journal of physiology. Heart and circulatory physiology 2021-04-16 [PMID: 33861145]
• Ge X, Zhang T, Yu X et al. LIM-Nebulette Reinforces Podocyte Structural Integrity by Linking Actin and Vimentin Filaments J. Am. Soc. Nephrol. 2020-07-31 [PMID: 32737144]
• Licker V, Turck N, Kovari E et al. Proteomic analysis of human substantia nigra identifies novel candidates involved in Parkinson's disease pathogenesis. Proteomics 2014-02-18 [PMID: 24449343] (IHC-P, Human)
• Bonzo JR, Norris AA, Esham M, Moncman CL. The nebulette repeat domain is necessary for proper maintenance of tropomyosin with the cardiac sarcomere. Exp Cell Res 2008-11-15 [PMID: 18823973]

Bioinformatics

• Gene Symbol: NEBL
• Entrez:
  • 10529(Human)
  • 74103(Mouse)
• Uniprot:
  • NP_998734.1(Human)