NDUFV2 Antibody (PSH02-86)

Immunohistochemistry: NDUFV2 Antibody (PSH02-86) [NBP3-32640] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-NDUFV2 antibody (NBP3-32640) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: NDUFV2 Antibody (PSH02-86) [NBP3-32640] - Immunocytochemistry analysis of RAW264.7 cells labeling NDUFV2 with Rabbit anti-NDUFV2 antibody (NBP3-32640) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NDUFV2 antibody at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Western Blot: NDUFV2 Antibody (PSH02-86) [NBP3-32640] - Western blot analysis of NDUFV2 on different lysates with Rabbit anti-NDUFV2 antibody (NBP3-32640) at 1/2,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: Ramos cell lysate (20 µg/Lane) Lane 5: Raji cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (20 µg/Lane) Lane 7: A431 cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: RAW264.7 cell lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (20 µg/Lane) Lane 11: Mouse heart tissue lysate (40 µg/Lane) Lane 12: Rat heart tissue lysate (40 µg/Lane) Lane 13: Human kidney tissue lysate (40 µg/Lane) Lane 14: Mouse kidney tissue lysate (40 µg/Lane) Predicted band size: 27 kDa Observed band size: 24 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: PSH02-86
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

NDUFV2 Antibody (PSH02-86) Summary

• Description: Novus Biologicals Rabbit NDUFV2 Antibody (PSH02-86) (NBP3-32640) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human NDUFV2 aa 1-249 / 249. (Uniprot: P19404)
• Localization: Mitochondrion inner membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NDUFV2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000-1:5000
  • Western Blot 1:2000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NDUFV2 Antibody (PSH02-86)

• CI-24k
• mitochondrial respitory chain, 24 kD subunit
• NADH dehydrogenase (ubiquinone) flavoprotein 2 (24kD)
• NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
• NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
• NADH dehydrogenase ubiquinone flavoprotein 2, mitochondrial
• NADH-ubiquinone oxidoreductase 24 kDa subunit
• NADH-ubiquinone oxidoreductase flavoprotein 2
• nuclear-encoded mitochondrial NADH-ubiquinone reductase 24Kd subunit

Background

NDUFV2 is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFV2