NDUFV1 Antibody (4A7) - Azide and BSA Free

Western Blot: NDUFV1 Antibody (4A7) [H00004723-M01] - Analysis of NDUFV1 expression in transfected 293T cell line by NDUFV1 monoclonal antibody (M01), clone 4A7.Lane 1: NDUFV1 transfected lysate(50.8 KDa).Lane 2: Non-transfected lysate.

Western Blot: NDUFV1 Antibody (4A7) [H00004723-M01] - Western Blot analysis of NDUFV1 expression in A-431 ( Cat # L015V1 ).

Western Blot: NDUFV1 Antibody (4A7) [H00004723-M01] - Analysis of NDUFV1 over-expressed 293 cell line, cotransfected with NDUFV1 Validated Chimera RNAi ( Cat # H00004723-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFV1 monoclonal antibody (M01), clone 4A7 (Cat # H00004723-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: NDUFV1 Antibody (4A7) [H00004723-M01] - NDUFV1 monoclonal antibody (M01), clone 4A7. Analysis of NDUFV1 expression in PC-12.

ELISA: NDUFV1 Antibody (4A7) [H00004723-M01] - Detection limit for recombinant GST tagged NDUFV1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Mycoplasma
• Clone: 4A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NDUFV1 Antibody (4A7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NDUFV1 (NP_002466, 365 a.a. ~ 464 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KAIARLIEFYKHESCGQCTPCREGVDWMNKVMARFVRGDARPAEIDSLWEISKQIEGHTICALGDGAAWPVQGLIRHFRPELEERMQRFAQQHQARQAAS
• Specificity: NDUFV1 (4A7)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NDUFV1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.
• Control:

  A-431 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NDUFV1 Antibody (4A7) - Azide and BSA Free

• CI-51K
• CI51KD
• CI-51kD
• complex I 51kDa subunit
• complex I, mitochondrial respiratory chain
• Complex I-51kD
• EC 1.6.5.3
• EC 1.6.99.3
• EC 1.6.99.5
• FLJ59059
• mitochondrial NADH dehydrogenase ubiquinone flavoprotein 1
• NADH dehydrogenase (ubiquinone) flavoprotein 1 (51kD)
• NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
• NADH dehydrogenase flavoprotein 1
• NADH-ubiquinone oxidoreductase 51 kDa subunit
• UQOR1NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

Background

The NDUFV1 gene encodes the 51-kD subunit of complex I (NADH:ubiquinone oxidoreductase) of the mitochondrial respiratory chain.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NDUFV1 Antibody (H00004723-M01)(1)

Publication using H00004723-M01

• Sheftel AD, Stehling O, Pierik AJ et al. Human Ind1, an iron-sulfur cluster assembly factor for respiratory complex I. Mol Cell Biol. 2009-09-14 [PMID: 19752196]

Bioinformatics

• Gene Symbol: NDUFV1
• Entrez:
  • 4723(Human)
• OMIM:
  • 161015(Human)
• Uniprot:
  • NP_009034.2(Human)