Ndufs1 Antibody - BSA Free

Western Blot: Ndufs1 Antibody [NBP1-31142] - Effect of BGP-15 treatment on the electron transport chain complex I and III proteins in NRCMs. Western blot analysis of UQCRC1 (NBP2-03825) and Ndufs1 (NBP1-31142) proteins is shown. GAPDH was used as a loading control. Control group: cells without any treatment; BGP-15 group: cells with only 50uM BGP-15 for 0.5 hours; H2O2 group: cells with 150uM H2O2 for 0.5 hours; H2O2+BGP-15 group: cells with 150uM H2O2and 50uM BGP-15 for 0.5 hours.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33728024/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Ndufs1 Antibody [NBP1-31142] - Ovarian carcinoma. NDUFS1 antibody dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: Ndufs1 Antibody [NBP1-31142] - 50 ug mouse brain lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:500.

Western Blot: Ndufs1 Antibody [NBP1-31142] - Hep G2 7.5% SDS PAGE diluted at 1:1500

Western Blot: Ndufs1 Antibody [NBP1-31142] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:10000.

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Ndufs1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Ndufs1 Antibody - BSA Free (NBP1-31142) is a polyclonal antibody validated for use in IHC and WB. Anti-Ndufs1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Ndufs1. The exact sequence is proprietary.
• Localization: Mitochondrion inner membrane
• Predicted Species: Bovine (96%), Chimpanzee (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFS1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (82%), Chicken (86%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Ndufs1 Antibody - BSA Free

• CI-75k
• CI-75kD
• complex I 75kDa subunit
• complex I, mitochondrial respiratory chain, 75-kD subunit
• Complex I-75kD
• EC 1.6.5.3
• EC 1.6.99.3
• EC 7.1.1.2
• MGC26839
• mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit
• NADH dehydrogenase (ubiquinone) Fe-S protein 1 (75kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Qreductase)
• NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
• Ndufs1
• PRO1304

Background

The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Ndufs1 Antibody (NBP1-31142)(2)

Publications using NBP1-31142

• Horvath O, Ordog K, Bruszt K et al. Modulation of Mitochondrial Quality Control Processes by BGP-15 in Oxidative Stress Scenarios: From Cell Culture to Heart Failure Oxidative medicine and cellular longevity 2021-02-27 [PMID: 33728024] (WB, Rat)
• Ordog K, Horvath O, Eros K, et al. Mitochondrial protective effects of PARP-inhibition in hypertension-induced myocardial remodeling and in stressed cardiomyocytes Life sciences 2021-01-06 [PMID: 33421523] (WB, Rat)

Bioinformatics

• Gene Symbol: NDUFS1