Ndufs1 Antibody

Images

 
Western Blot: Ndufs1 Antibody [NBP1-31142] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:10000.
Immunohistochemistry-Paraffin: Ndufs1 Antibody [NBP1-31142] - Paraffin-embedded ovarian carcinoma. NDUFS1 antibody dilution: 1:250.
Western Blot: Ndufs1 Antibody [NBP1-31142] - 50 ug mouse brain lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:500.
Western Blot: Ndufs1 Antibody [NBP1-31142] - Hep G2 7.5% SDS PAGE diluted at 1:1500

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, MkSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Ndufs1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NDUFS1. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane
Predicted Species
Bovine (96%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NDUFS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (82%), Chicken (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Ndufs1 Antibody

  • CI-75k
  • CI-75Kd
  • complex I 75kDa subunit
  • complex I, mitochondrial respiratory chain, 75-kD subunit
  • Complex I-75kD
  • EC 1.6.5.3
  • EC 1.6.99.3
  • MGC26839
  • mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit
  • NADH dehydrogenase (ubiquinone) Fe-S protein 1 (75kD) (NADH-coenzyme Qreductase)
  • NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Qreductase)
  • NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
  • PRO1304

Background

The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol NDUFS1