Ndufs1 Antibody (S02-3I2)

Western Blot: Ndufs1 Antibody (S02-3I2) [NBP3-19894] - Western blot detection of Ndufs1 in Hela, A549, HL-60 using NBP3-19894 (1:1000 diluted)

Immunohistochemistry-Paraffin: Ndufs1 Antibody (S02-3I2) [NBP3-19894] - Immunohistochemistry of Ndufs1 in paraffin-embedded Human Cholangiocarcinoma using NBP3-19894 at dilution 1/50

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: S02-3I2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Ndufs1 Antibody (S02-3I2) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Ndufs1 (Uniprot # P28331)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NDUFS1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000-1/10000
• Theoretical MW: 77 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Ndufs1 Antibody (S02-3I2)

• CI-75k
• CI-75kD
• complex I 75kDa subunit
• complex I, mitochondrial respiratory chain, 75-kD subunit
• Complex I-75kD
• EC 1.6.5.3
• EC 1.6.99.3
• EC 7.1.1.2
• MGC26839
• mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit
• NADH dehydrogenase (ubiquinone) Fe-S protein 1 (75kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Qreductase)
• NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
• Ndufs1
• PRO1304

Background

Ndufs1 is encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFS1