NDUFA5 Antibody - BSA Free

Western Blot: NDUFA5 Antibody [NBP1-33504] - Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: NDUFA5 Antibody [NBP1-33504] - NDUFA5 antibody detects NDUFA5 protein at mitochondria in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. NDUFA5 antibody diluted at 1:500.

Western Blot: NDUFA5 Antibody [NBP1-33504] - Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: NDUFA5 Antibody [NBP1-33504] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NDUFA5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NDUFA5 Antibody - BSA Free (NBP1-33504) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human NDUFA5 Recombinant protein.
• Localization: Mitochondrion inner membrane, Peripheral membrane protein, Matrix side
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFA5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chimpanzee (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7)
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for NDUFA5 Antibody - BSA Free

• B13,5 (13kD, B13)
• DKFZp781K1356
• NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa
• UQOR13FLJ12147

Background

The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NDUFA5