NDUFA12 Antibody - BSA Free

Immunohistochemistry-Paraffin: NDUFA12 Antibody [NBP1-32745] - NDUFA12 Antibody detects NDUFA12 protein at mitochondria in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. NDUFA12 Antibody diluted at 1:500.

Immunohistochemistry-Paraffin: NDUFA12 Antibody [NBP1-32745] - Cal27 xenograft, using NDUFA12 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunocytochemistry/Immunofluorescence: NDUFA12 Antibody [NBP1-32745] - Immunofluorescence analysis of methanol-fixed HepG2, using NDUFA12 antibody at 1:200 dilution.

Western Blot: NDUFA12 Antibody [NBP1-32745] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA12 antibody [N1C3] (NBP1-32745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: NDUFA12 Antibody [NBP1-32745] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA12 antibody [N1C3] (NBP1-32745) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NDUFA12 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NDUFA12 Antibody - BSA Free (NBP1-32745) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human NDUFA12 Recombinant protein.
• Localization: Mitochondrion inner membrane, Peripheral membrane protein, Matrix side
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDUFA12
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for NDUFA12 Antibody - BSA Free

• 13 kDa differentiation-associated protein
• B17.2,13kDa differentiation-associated protein
• CIB17.2
• CI-B17.2
• complex I B17.2 subunit
• Complex I-B17.2
• DAP13NADH-ubiquinone oxidoreductase subunit B17.2
• NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12
• NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
• NADH: ubiquinone oxidoreductase

Background

DAP13 transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NDUFA12