midA Antibody (OTI4A5)

Images

 
Western Blot: midA Antibody (4A5) [NBP2-00820] Analysis of extracts (35ug) from 9 different cell lines by using anti-midA monoclonal antibody.
Immunohistochemistry-Paraffin: midA Antibody (4A5) [NBP2-00820] - Staining of paraffin-embedded Human tonsil using anti-midA mouse monoclonal antibody.
Western Blot: midA Antibody (4A5) [NBP2-00820] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY midA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: midA Antibody (4A5) [NBP2-00820] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-midA mouse monoclonal antibody.
Immunohistochemistry-Paraffin: midA Antibody (4A5) [NBP2-00820] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-midA mouse monoclonal antibody.
Immunohistochemistry-Paraffin: midA Antibody (4A5) [NBP2-00820] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-midA mouse monoclonal antibody.
Immunohistochemistry-Paraffin: midA Antibody (4A5) [NBP2-00820] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-midA mouse monoclonal antibody.
Immunohistochemistry-Paraffin: midA Antibody (4A5) [NBP2-00820] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-midA mouse monoclonal antibody.
Immunohistochemistry-Paraffin: midA Antibody (4A5) [NBP2-00820] - Staining of paraffin-embedded Human breast tissue using anti-midA mouse monoclonal antibody.
Immunohistochemistry-Paraffin: midA Antibody (4A5) [NBP2-00820] - Staining of paraffin-embedded Human Kidney tissue using anti-midA mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
OTI4A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.86 mg/ml

midA Antibody (OTI4A5) Summary

Immunogen
Full length human recombinant protein of human C2orf56(NP_653337) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NDUFAF7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
49.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.86 mg/ml
Purity
Immunogen affinity purified

Alternate Names for midA Antibody (OTI4A5)

  • chromosome 2 open reading frame 56
  • MidA
  • mitochondrial
  • PRO1853

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NDUFAF7
Entrez