NDUFA10 Antibody

Images

 
Western Blot: NDUFA10 Antibody [NBP1-32146] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NDUFA10 antibody [N1C3] diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: NDUFA10 Antibody [NBP1-32146] - HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37(c) for 30 min.Green: NDUFA10 protein stained by NDUFA10 antibody [N1C3] diluted ...read more
Immunohistochemistry-Paraffin: NDUFA10 Antibody [NBP1-32146] - Paraffin-embedded colon, using antibody at 1:100 dilution.
Western Blot: NDUFA10 Antibody [NBP1-32146] - Sample(30 ug of whole cell lysate)A:H1299 B:Raji 10% SDS PAGE, antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NDUFA10 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NDUFA10. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Predicted Species
Primate (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NDUFA10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-32146 in the following applications:

Reactivity Notes

Chimpanzee (99%), Bovine (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for NDUFA10 Antibody

  • 10 (42kD)
  • CI-42k
  • CI-42KD
  • FLJ21923
  • MGC5103
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
  • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

Background

The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Review for NDUFA10 Antibody (NBP1-32146) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-32146:
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WB
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Sven Dennerlein
WB Human 03/05/2014
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Summary

ApplicationWestern Blot
Sample TestedHEK293 cells
SpeciesHuman

Blocking

Blocking Details1 h, at room temperature in 5 % milk

Primary Anitbody

Dilution Ratio1:500, can go down to 1:2000

Secondary Antibody

Secondary Descriptionanti rabbit
Secondary Concentration1:10000

Details

Detection NotesX-ray films, exposure app. 10 - 60 sec

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NDUFA10 Antibody (NBP1-32146) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Diseases for NDUFA10 Antibody (NBP1-32146)

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Sven Dennerlein
03/05/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol NDUFA10