NDRG2 Antibody

Western Blot: NDRG2 Antibody [NBP2-76951] - Western blot analysis of NDRG2 on mouse brain (1) and mouse skeletal muscle (2) tissue lysate using anti-NDRG2 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: NDRG2 Antibody [NBP2-76951] - ICC staining NDRG2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: NDRG2 Antibody [NBP2-76951] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NDRG2 antibody. Counter stained with hematoxylin.

Flow Cytometry: NDRG2 Antibody [NBP2-76951] - Flow cytometric analysis of HepG2 cells with NDRG2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: NDRG2 Antibody [NBP2-76951] - ICC staining NDRG2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: NDRG2 Antibody [NBP2-76951] - ICC staining NDRG2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: NDRG2 Antibody [NBP2-76951] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NDRG2 antibody. Counter stained with hematoxylin.

Immunohistochemistry: NDRG2 Antibody [NBP2-76951] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NDRG2 antibody. Counter stained with hematoxylin.

Immunohistochemistry: NDRG2 Antibody [NBP2-76951] - Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NDRG2 antibody. Counter stained with hematoxylin.

Product Discontinued

NDRG2 Antibody Summary

• Immunogen: Recombinant protein within Human NDRG2 aa 13-160 / 371. (SwissProt: Q9UN36 Human; SwissProt: Q9QYG0 Mouse; SwissProt: Q8VBU2 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NDRG2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NDRG2 Antibody

• DKFZp781G1938
• FLJ25522
• KIAA1248cytoplasmic protein Ndr1
• NDR1-related protein NDR2
• NDRG family member 2
• protein NDRG2
• Protein Syld709613
• syld709613 protein
• SYLDN-myc downstream regulator 2

Background

NDRG2 is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDRG2