NDRG1 Antibody (NDRG1/1383)

Western Blot: NDRG1 Antibody (NDRG1/1383) [NBP2-48475] - Analysis of Kidney and HeLa Cell Lysate using NDRG1 Monoclonal Antibody (NDRG1/1383)

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NDRG1 Antibody (NDRG1/1383) Summary

• Immunogen: Recombinant fragment of human NDRG1 protein
• Localization: Cytoplasmic and Cell Surface
• Marker: Marker of Tumor Aggressiveness
• Specificity: It recognizes a protein of 43kDa, which is identified as N-myc downstream-regulated gene 1 protein (NDRG1). The NDRG) family is comprised of four members, NDRG1, NDRG2, NDRG3 and NDRG4, which share 57-65% homology. The NDRG1 gene is ubiquitously expressed, but it is expressed most prominently in placental membranes and prostate, kidney, small intestine and ovary tissue. NDRG1 is a direct transcriptional target gene of p53 to mediated cell death and apoptosis. NDRG1 gene expression is induced by several compounds, including nickel, and produces a protein involved in stress responses, hormone responses, cell growth and differentiation. The reduced expression of NDRG1 has been found to be associated with tumor metastasis in a variety of tumors, including cancers of the breast, colon, prostate, oral cavity and oropharynx. Reportedly, overexpression of NDRG1 in hepatocellular carcinoma is an indicator of tumor aggressiveness.
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NDRG1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/ Immunofluorescence 0.5-1ug/ml
  • Western Blot 0.5-1ug/ml
• Application Notes: Human Chromosome Location: 8q24.3
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for NDRG1 Antibody (NDRG1/1383)

• CAP43
• CMT4D
• Differentiation-related gene 1 protein
• DRG1
• DRG-1
• DRG1HMSNL
• GC4
• HMSNL
• NDR1
• NDRG1
• Nickel-specific induction protein Cap43
• NMSL
• N-myc downstream regulated 1
• N-myc downstream-regulated gene 1 protein
• protein NDRG1
• protein regulated by oxygen-1
• PROXY1
• Reducing agents and tunicamycin-responsive protein
• RIT42
• RTP
• RTPGC4
• TARG1
• TDD5
• tunicamycin-responsive protein

Background

NDRG1 is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for NDRG1 Antibody (NBP2-48475). (Showing 1 - 1 of 1 FAQ).

1. I am planing to detect and sort human cells which express NDRG1 or TrkB on cell surface by these antibodies via FACS. I'm looking at the following catalog numbers: NBP2-54550PCP, NBP2-52523PCP and NBP2-52524PCP. In that case, are the epitopes that these antibodies react to located on cell surface and possible to be used as cell surface markers in FACS?
   • Since all of these antibodies are validated in FLOW for human they will be guaranteed to work for you. For the NDRG1 we find that it will be intracellular and located in the cytoplasm and cell surface so it should be accessible for FLOW.http://www.proteinatlas.org/ENSG00000104419-NDRG1/tissueNBP2-54550PCP NDRG1 Antibody (NDRG1/1383) PCPImmunogen - Recombinant fragment of human NDRG1 fragment (aa162-283) (exact sequence is proprietary)Localization -Cytoplasmic and Cell SurfaceFor NTRK2 we find it is membrane and cytoplasmic and the epitopes of the antibodies below are in the extracelluar domain.http://www.proteinatlas.org/ENSG00000148053-NTRK2/tissueNBP2-52523PCP TrkB Antibody (8D2E8)Immunogen-Purified recombinant fragment of human NTRK2 (AA: 207-339) expressed in E. Coli.Therefore the epitope is located in the Topological domain= 32 – 430 ExtracellularNBP2-52524PCP TrkB Antibody (10B6C4) is the same immunogen just a different clone

Bioinformatics

• Gene Symbol: NDRG1