![Western Blot: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of NDRG1 on SW480 cell (1) and mouse eye tissue (2) lysate using anti-NDRG1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize2/NDRG1 Antibody (JM32-02)-Western Blot-NBP2-66969-img0007.jpg "Western Blot: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of NDRG1 on SW480 cell (1) and mouse eye tissue (2) lysate using anti-NDRG1 antibody at 1/1,000 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P, IP |
| Clone | JM32-02 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein |
| Localization | Cytoplasm. Nucleus. Cell membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | NDRG1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1%BSA, 40%Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publication using NBP2-66969 | Applications | Species |
|---|---|---|
| Ortega FG, Roefs MT, de Miguel Perez D et al. Interfering with endolysosomal trafficking enhances release of bioactive exosomes Nanomedicine May 30 2019 [PMID: 31152797] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Diseases for NDRG1 Antibody (NBP2-66969)Discover more about diseases related to NDRG1 Antibody (NBP2-66969).
| Pathways for NDRG1 Antibody (NBP2-66969)View related products by pathway.
|
PTMs for NDRG1 Antibody (NBP2-66969)Learn more about PTMs related to NDRG1 Antibody (NBP2-66969).
| Research Areas for NDRG1 Antibody (NBP2-66969)Find related products by research area.
|
|
Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NDRG1 |
![Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (JM32-02) [NBP2-66969] - Staining NDRG1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/NDRG1-Antibody-(JM32-02)-Immunocytochemistry-Immunofluorescence-NBP2-66969-img0002.jpg "Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (JM32-02) [NBP2-66969] - Staining NDRG1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of paraffin-embedded mouse kidney tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/NDRG1-Antibody-(JM32-02)-Immunohistochemistry-Paraffin-NBP2-66969-img0006.jpg "Immunohistochemistry-Paraffin: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of paraffin-embedded mouse kidney tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.")
![Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (JM32-02) [NBP2-66969] - Staining NDRG1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/NDRG1-Antibody-(JM32-02)-Immunocytochemistry-Immunofluorescence-NBP2-66969-img0001.jpg "Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (JM32-02) [NBP2-66969] - Staining NDRG1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of paraffin-embedded human colon cancer tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/NDRG1-Antibody-(JM32-02)-Immunohistochemistry-Paraffin-NBP2-66969-img0003.jpg "Immunohistochemistry-Paraffin: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of paraffin-embedded human colon cancer tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of paraffin-embedded human placenta tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/NDRG1-Antibody-(JM32-02)-Immunohistochemistry-Paraffin-NBP2-66969-img0004.jpg "Immunohistochemistry-Paraffin: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of paraffin-embedded human placenta tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of paraffin-embedded human prostate tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/NDRG1-Antibody-(JM32-02)-Immunohistochemistry-Paraffin-NBP2-66969-img0005.jpg "Immunohistochemistry-Paraffin: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of paraffin-embedded human prostate tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.")
![Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in IMR32 whole cell lysate.](http://images.novusbio.com/images2/n-Myc-Antibody-(NMYC-1)-Western-Blot-NB200-109-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: n-Myc Antibody (NMYC-1) [NB200-109] - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/images2/n-Myc-Antibody-(NMYC-1)-Immunocytochemistry--Immunofluorescence-NB200-109-img0003.jpg)
![Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Analysis of n-Myc expression in human heart tissue.](http://images.novusbio.com/images2/n-Myc-Antibody-(NMYC-1)-Western-Blot-NB200-109-img0001.jpg)
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. WB image submitted by a verified customer review.](http://images.novusbio.com/images2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-105-img0007.jpg)
![Knockout Validated: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog # NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using a NorthernLights (TM) 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/images2/HIF-1-alpha-Antibody-(H1alpha67)-Knockout-Validated-NB100-105-img0027.jpg)
![Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1 alpha and Lamin B1 levels were analyzed by Western blot. Image from verified customer review.](http://images.novusbio.com/images2/HIF-1-alpha-Antibody-(H1alpha67)-Western-Blot-NB100-105-img0026.jpg)
![Western Blot: STK38L Antibody (OTI4D8) - Azide and BSA Free [NBP2-74403] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK38L.](http://images.novusbio.com/images2/STK38L-Antibody-(OTI4D8)---Azide-and-BSA-Free-Western-Blot-NBP2-74403-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: STK38L Antibody (OTI4D8) - Azide and BSA Free [NBP2-74403] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY STK38L.](http://images.novusbio.com/images2/STK38L Antibody (OTI4D8) - Azide and BSA Free-Immunocytochemistry-Immunofluorescence-NBP2-74403-img0008.jpg)
![Immunohistochemistry: STK38L Antibody (OTI4D8) - Azide and BSA Free [NBP2-74403] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/STK38L-Antibody-(OTI4D8)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-74403-img0007.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).](http://images.novusbio.com/images2/p21-CIP1-CDKN1A-Antibody-(WA-1-(HJ21))-Immunohistochemistry-Paraffin-NBP2-29463-img0001.jpg)
![Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/images2/c-Myc-Antibody-Western-Blot-NB600-335-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the Alexa Fluor 488 format of this antibody.](http://images.novusbio.com/images2/c-Myc-Antibody-Immunocytochemistry-Immunofluorescence-NB600-335-img0007.jpg)
![Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).](http://images.novusbio.com/images2/c-Myc-Antibody-Western-Blot-NB600-335-img0005.jpg)
![Western Blot: STK38 Antibody (2G8-1F3) [H00011329-M01] - STK38 monoclonal antibody (M01), clone 2G8-1F3. Analysis of STK38 expression in human kidney.](http://images.novusbio.com/images2/STK38-Antibody-(2G8-1F3)-Western-Blot-H00011329-M01-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: STK38 Antibody (2G8-1F3) [H00011329-M01] - Analysis of monoclonal antibody to STK38 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/STK38-Antibody-(2G8-1F3)-Immunocytochemistry-Immunofluorescence-H00011329-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: STK38 Antibody (2G8-1F3) [H00011329-M01] - Analysis of monoclonal antibody to STK38 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue Antibody concentration 5 ug/ml.](http://images.novusbio.com/images2/STK38-Antibody-(2G8-1F3)-Immunohistochemistry-Paraffin-H00011329-M01-img0009.jpg)
![Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi](http://images.novusbio.com/images2/Androgen-R-NR3C4-[p-Ser213--p-Ser210]-Antibody-(156C135.2)-Western-Blot-NB100-56603-img0004.jpg)
![Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Western-Blot-NBP2-37576-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Immunocytochemistry-Immunofluorescence-NBP2-37576-img0004.jpg)
![Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.](http://images.novusbio.com/images2/c-jun-Antibody-(5B1)-Immunohistochemistry-NBP2-37576-img0007.jpg)
![Knockdown Validated: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 downregulates GLUT1 by promoting its ubiquitination. ((B), (D) and (F) T-47D cells were transfected with NDRG2 small interfering RNA (siRNA) 10, 25 and 100 pmol or control siRNA for 48 hours. Next, cell proteins or mRNA were extracted and analysed by immunoblotting (B). beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (http://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NDRG2-Antibody-(6A5)-Western-Blot-H00057447-M03-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 interacts with GLUT1. SK-BR-3 cells were fixed and incubated with primary antibodies against N-myc downstream-regulated gene 2 (NDRG2) or glucose transporter 1 (GLUT1) and with fluorescein isothiocyanate or a cyanine 3 secondary antibody. Green fluorescence indicates NDRG2 expression, red fluorescence indicates GLUT1 expression and blue fluorescence indicates nuclear staining. The results of the merged images reveal that NDRG2 and GLUT1 were colocalised in the cytoplasm. Image collected and cropped by CiteAb from the following publication (http://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NDRG2-Antibody-(6A5)-Immunocytochemistry-Immunofluorescence-H00057447-M03-img0005.jpg)
![Immunohistochemistry: NDRG2 Antibody (6A5) [H00057447-M03] - NDRG2 is correlated with increased survival and negatively correlated with GLUT1 in breast carcinoma. Kaplan-Meier analysis was carried out according to N-myc downstream-regulated gene 2 (NDRG2) expression levels of disease-free survival. Serial immunostained sections for NDRG2 and glucose transporter 1 (GLUT1) in breast cancer and normal tissues were analysed. Original magnification, 40x (top) and 400x (bottom); scale bars = 50 um. Image collected and cropped by CiteAb from the following publication (http://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3628) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NDRG2-Antibody-(6A5)-Immunohistochemistry-H00057447-M03-img0007.jpg)
![Knockdown Validated: YB1 Antibody [NBP1-97572] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YB-1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:10000 ug/ml of Rabbit Anti-Human YB-1 Polyclonal Antibody (Catalog # NBP1-97572) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for YB-1 at approximately 49 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/YB1-Antibody-Knockdown-Validated-NBP1-97572-img0007.jpg)
![Western Blot: YB1 Antibody [NBP1-97572] - Western blot analysis of YB1 expression in 1) HeLa and 2) NTera2 whole cell lysates using NBP1-97572.](http://images.novusbio.com/images/YB1-Antibody-Western-Blot-NBP1-97572-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: YB1 Antibody [NBP1-97572] - The YB1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin (NB100-690) were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/images2/YB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97572-img0004.jpg)
![Western Blot: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of SGK1 on human kidney lysates using anti-SGK1 antibody at 1/1,000 dilution.](http://images.novusbio.com/images2/SGK1 Antibody (SC05-71)-Western Blot-NBP2-67561-img0007.jpg)
![Immunohistochemistry-Paraffin: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of paraffin-embedded mouse liver tissue using anti-SGK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/SGK1-Antibody-(SC05-71)-Immunohistochemistry-Paraffin-NBP2-67561-img0006.jpg)
![Flow Cytometry: SGK1 Antibody (SC05-71) [NBP2-67561] - Analysis of 293 cells with SGK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/images2/SGK1-Antibody-(SC05-71)-Flow-Cytometry-NBP2-67561-img0001.jpg)
![Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/images2/HYPB-Antibody-(CL9956)-Immunohistochemistry-Paraffin-NBP2-88941-img0004.jpg)
![Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/images2/HYPB-Antibody-(CL9956)-Immunohistochemistry-Paraffin-NBP2-88941-img0001.jpg)
![Immunohistochemistry-Paraffin: HYPB Antibody (CL9956) [NBP2-88941] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/images2/HYPB-Antibody-(CL9956)-Immunohistochemistry-Paraffin-NBP2-88941-img0002.jpg)
![Western Blot: NDRG3 Antibody [NBP1-86046] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/images2/NDRG3 Antibody-Western Blot-NBP1-86046-img0005.jpg)
![Immunohistochemistry-Paraffin: NDRG3 Antibody [NBP1-86046] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/NDRG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86046-img0001.jpg)
![Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Images showing Carbonic Anhydrase IX/CA9 expression, pimonidazole, apoptosis and proliferation in SCCNij202.Carbonic Anhydrase IX/CA9 is expressed in hypoxic regions as assessed by pimonidazole staining (A), but is also observed in non-pimonidazole areas (B). Yet, most of Carbonic Anhydrase IX/CA9 is expressed in pimonidazole positive regions (C). Proliferation (BrdUrd) and apoptosis (caspase-3) in relation to Carbonic Anhydrase IX/CA9 expression are shown in figure D and E. Red, Carbonic Anhydrase IX/CA9; Green, pimonidazole (A-B), BrdUrd (D) or caspase-3 (E); Yellow, overlap of Carbonic Anhydrase IX/CA9 (red) and pimonidazole (green); Light blue, vessels. Magnification 100x. Scale bars represent 100 um. Closed circles represent Carbonic Anhydrase IX/CA9 expression in pimonidazole positive regions; open circles represent Carbonic Anhydrase IX/CA9 expression in pimonidazole negative regions. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0108068), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Carbonic-Anhydrase-IX-CA9-Antibody-Immunohistochemistry-NB100-417-img0047.jpg)
![Dual RNAscope ISH-IHC: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for Carbonic Anhydrase IX/CA9 mRNA (ACD RNAScope Probe, catalog # 559348; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus catalog # NB100-417) at 1:1000 dilution with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.](http://images.novusbio.com/images2/Carbonic-Anhydrase-IX-CA9-Antibody-Dual-RNAscope-ISH-IHC-NB100-417-img0041.jpg)
![Western Blot: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis on various human cell lysates. Specific bands were detected for Carbonic Anhydrase IX/CA9 in HEK-293 and A498 cell lines at a molecular weight of approximately 50 kDa. WB image submitted by a verified customer review.](http://images.novusbio.com/images2/Carbonic-Anhydrase-IX-CA9-Antibody-Western-Blot-NB100-417-img0013.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")