NDRG1 Antibody (JM32-02)

Western Blot: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of NDRG1 on SW480 cell (1) and mouse eye tissue (2) lysate using anti-NDRG1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (JM32-02) [NBP2-66969] - Staining NDRG1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of paraffin-embedded mouse kidney tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: NDRG1 Antibody (JM32-02) [NBP2-66969] - Staining NDRG1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of paraffin-embedded human colon cancer tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of paraffin-embedded human placenta tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NDRG1 Antibody (JM32-02) [NBP2-66969] - Analysis of paraffin-embedded human prostate tissue using anti-NDRG1 antibody. Counter stained with hematoxylin.

Product Discontinued

NDRG1 Antibody (JM32-02) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human NDRG1 aa 345-394 / 394. (SwissProt: Q92597 Human; SwissProt: Q62433 Mouse; SwissProt: Q6JE36 Rat)
• Localization: Cytoplasm. Nucleus. Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NDRG1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NDRG1 Antibody (JM32-02)

• CAP43
• CMT4D
• Differentiation-related gene 1 protein
• DRG1
• DRG-1
• DRG1HMSNL
• GC4
• HMSNL
• NDR1
• NDRG1
• Nickel-specific induction protein Cap43
• NMSL
• N-myc downstream regulated 1
• N-myc downstream-regulated gene 1 protein
• protein NDRG1
• protein regulated by oxygen-1
• PROXY1
• Reducing agents and tunicamycin-responsive protein
• RIT42
• RTP
• RTPGC4
• TARG1
• TDD5
• tunicamycin-responsive protein

Background

NDRG1 is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NDRG1 Antibody (NBP2-66969)(2)

Publications using NBP2-66969

• Cheng Q, Ning S, Zhu L et al. NDRG1 facilitates self-renewal of liver cancer stem cells by preventing EpCAM ubiquitination British journal of cancer 2023-05-10 [PMID: 37165202] (IHC, Human)
• Ortega FG, Roefs MT, de Miguel Perez D et al. Interfering with endolysosomal trafficking enhances release of bioactive exosomes Nanomedicine 2019-05-30 [PMID: 31152797] (WB, Human)

Bioinformatics

• Gene Symbol: NDRG1