NDE1 Overexpression Lysate (Native)


Western Blot: NDE1 Overexpression Lysate (Adult Normal) [NBL1-13522] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NDE1.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

NDE1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC200179 and based on accession number NM_017668. The protein contains a C-terminal DDK tag.
Homo sapiens nudE nuclear distribution gene E homolog 1 (A. nidulans) (NDE1), transcript variant 2, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
37.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for NDE1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NDE1 Overexpression Lysate (Native)

  • FLJ20101
  • HOM-TES-87
  • nuclear distribution protein nudE homolog 1
  • nudE nuclear distribution gene E homolog 1 (A. nidulans)
  • NudE
  • NUDE1
  • rat homolog


This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for NDE1 Lysate (NBL1-13522) (0)

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Product PDFs


Gene Symbol NDE1

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