NCOA3/AIB1 Antibody (AC3)

Product Discontinued

NCOA3/AIB1 Antibody (AC3) Summary

• Immunogen: A synthetic peptide to the C-terminal end of human AIB1 conjugated to KLH.
• Specificity: NB200-301 is specific for AIB1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NCOA3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1-5 ug/ml
• Theoretical MW: 160 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for NCOA3/AIB1 Antibody (AC3)

• ACTR
• ACTRCBP-interacting protein
• AIB1
• AIB1AIB-1
• Amplified in breast cancer 1 protein
• BHLHE42
• bHLHe42p/CIP
• CAGH16
• Class E basic helix-loop-helix protein 42
• CTG26
• KAT13B
• MGC141848
• NCOA3
• NCoA-3
• nuclear receptor coactivator 3
• pCIP
• pCIP
• RAC3
• RAC-3
• RAC3pCIP
• Receptor-associated coactivator 3
• SRC-1
• SRC3
• SRC-3
• SRC-3EC 2.3.1.48
• Steroid receptor coactivator protein 3
• Thyroid hormone receptor activator molecule 1
• TNRC16
• TRAM1
• TRAM-1TNRC14

Background

NCOA3/SRC3 (nuclear receptor coactivator 3/steroid receptor coactivator 3 (SRC3)) is a 155 kD member of the p160/steroid receptor coactivator family containing a nuclear localization sequence, bHLH, and PAS domain. There are two reported isoforms of this protein. NCOA3/SRC3 is localized mainly in the cytoplasm, with weak nuclear expression. The protein is translocated to the nucleus upon phosphorylation. NCOA3/SRC3 is widely expressed with high expression in the heart, skeletal muscle, pancreas, and placenta and low expression in the brain. NCOA3/SRC3 is expressed at very low levels in the lung, liver, and kidney and overexpressed in breast and ovarian cancers. This protein has histone acetyltransferase activity and functions as a transcriptional coactivator for steroid and nuclear hormone receptors. Phosphorylation by I kappaB kinase enhances coactivator function. Acetylation by CREB-BP disrupts interaction with nuclear receptors. NCOA3/SRC3 forms a multisubunit coactivation complex with NCOA2 IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein and has also been shown to interact with PCAF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCOA3/AIB1 Antibody (NB200-301)(2)

Publications using NB200-301

• Harigopal M, Heymann J, Ghosh S et al. Estrogen receptor co-activator (AIB1) protein expression by automated quantitative analysis (AQUA) in a breast cancer tissue microarray and association with patient outcome. Breast Cancer Res Treat 2009-05-01 [PMID: 18521745] (IF/IHC, ICC/IF, Human)
• Nakuci E, Mahner S, Direnzo J et al. BRCA1-IRIS regulates cyclin D1 expression in breast cancer cells. Exp Cell Res 2006-10-01 [PMID: 16860316] (Human)

Bioinformatics

• Gene Symbol: NCOA3
• Entrez:
  • 8202(Human)