NCF4 Antibody

Western Blot: NCF4 Antibody [NB100-61666] - HEK293 overexpressing P40PHOX and probed with (mock transfection in first lane).

Immunohistochemistry-Paraffin: NCF4 Antibody [NB100-61666] - Staining of paraffin embedded Human Spleen at dilution of 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: NCF4 Antibody [NB100-61666] - Analysis of NCF4 antibody using NB100-61666 at 0.01ug/ml in Daudi cell lysate (35ug protein in RIPA buffer).

Immunohistochemistry-Paraffin: NCF4 Antibody [NB100-61666] - (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NCF4 Antibody Summary

• Immunogen: Peptide with sequence C-KDFPEEDDPTN corresponding to internal region according to NP_000622.2, NP_038202.1.
• Epitope: KDFPEEDDPTN
• Specificity: This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NCF4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 38 kDa band observed in Daudi lysates (calculated MW of 39.0 kDa band according to NP_000622.2 and 38.8 kDa band according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human tonsil shows staining of the cytoplasm in some cells.
• Control:

  NCF4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NCF4 Antibody

• MGC3810
• NCF
• NCF-4
• neutrophil cytosol factor 4
• neutrophil cytosolic factor 4 (40kD)
• neutrophil cytosolic factor 4, 40kDa
• Neutrophil NADPH oxidase factor 4
• p40phox
• p40-phox
• SH3 and PX domain-containing protein 4
• SH3PXD4P40PHOX

Background

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCF4 Antibody (NB100-61666)(1)

Publication using NB100-61666

• Rioux JD, Xavier RJ, Taylor KD et al. Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis. Nat Genet 2007-05-01 [PMID: 17435756]

Bioinformatics

• Gene Symbol: NCF4
• Entrez:
  • 4689(Human)
• Uniprot:
  • NP_000622.2(Human)