NCF1 Antibody

Western Blot: NCF1 Antibody [NBP2-26113] - Staining of Daudi cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: NCF1 Antibody [NBP2-26113] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunocytochemistry/Immunofluorescence: NCF1 Antibody [NBP2-26113] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NCF1 Antibody Summary

• Description: Novus Biologicals Goat NCF1 Antibody (NBP2-26113) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-NCF1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DEAEDPDPNYAGEP corresponding to internal region according to NP_035006.3.
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NCF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/ml

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NCF1 Antibody

• 47 kDa neutrophil oxidase factor
• FLJ79451
• NADPH oxidase organizer 2
• NCF-1
• NCF1A
• neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein
• neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1)
• neutrophil cytosolic factor 1
• neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1)
• Neutrophil NADPH oxidase factor 1
• Nox organizer 2
• NOXO2NCF-47K
• Nox-organizing protein 2
• p47phox
• SH3 and PX domain-containing protein 1A
• SH3PXD1Ap47-phox

Background

NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1 are the cause of autosomal cytochrome-b-positive chronic granulomatous disease type 1 (CGD). NCF1 is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCF1 Antibody (NBP2-26113)(1)

Publication using NBP2-26113

• Roos D, Kuhns DB, Maddalena A et al. Hematologically important mutations: the autosomal recessive forms of chronic granulomatous disease (second update). Blood Cells Mol Dis 2010 Apr 15 [PMID: 20167518]

Bioinformatics

• Gene Symbol: NCF1
• Entrez:
  • 653361(Human)
  • 17969(Mouse)
  • 114553(Rat)