NCBP1 Antibody

Western Blot: NCBP1 Antibody [NBP2-33546] - Analysis using Anti-NCBP1 antibody NBP2-33546 (A) shows similar pattern to independent antibody NBP2-33897 (B).

Immunocytochemistry/Immunofluorescence: NCBP1 Antibody [NBP2-33546] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: NCBP1 Antibody [NBP2-33546] - Staining of liver cancer.

Immunohistochemistry-Paraffin: NCBP1 Antibody [NBP2-33546] - Staining of human duodenum shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: NCBP1 Antibody [NBP2-33546] - Staining of pancreas.

Product Discontinued

NCBP1 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: FSHHLSNFQFRWSWEDWSDCLSQDPESPKPKFVREVLEKCMRLSYHQRILDIVPPTFSALCPANPTCIYKYGDESSNSLPGHSVALCLAVAFKSKATNDEIFSILKDVPNPN
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NCBP1 Antibody

• CBP80nuclear cap binding protein subunit 1, 80kD
• NCBP 80 kDa subunit
• NCBPMGC2087
• nuclear cap binding protein subunit 1, 80kDa
• nuclear cap-binding protein subunit 1,80 kDa nuclear cap-binding protein
• Sto1

Background

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCBP1
• Uniprot:
  • Q09161(Human)