CBP20 Antibody - BSA Free

Western Blot: CBP20 Antibody [NBP1-30445] - Detection of Human and Mouse NCBP2 Whole cell lysate from HeLa and mouse NIH3T3 cells. NCBP2 was also immunoprecipitated by rabbit anti-NCBP2 antibody NBP1-30444.

Immunohistochemistry-Paraffin: CBP20 Antibody [NBP1-30445] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- NCBP2 used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1 ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Bv
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CBP20 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CBP20 Antibody - BSA Free (NBP1-30445) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 106 to 156 of human nuclear cap binding protein subunit 2 using the numbering given in entry NP_031388.2 (GeneID 22916).
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCBP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 5-15 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CBP20 Antibody - BSA Free

• 20 kDa nuclear cap-binding protein
• Cbc2
• CBP20CBC2
• NCBP 20 kDa subunit
• NCBP interacting protein 1
• NCBP-interacting protein 1
• NIP1Cell proliferation-inducing gene 55 protein
• nuclear cap binding protein subunit 2, 20kD
• nuclear cap binding protein subunit 2, 20kDa
• nuclear cap-binding protein subunit 2

Background

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCBP2
• Entrez:
  • 22916(Human)
• Uniprot:
  • P52298(Human)