NCAM-1/CD56 Antibody (2524C) [Unconjugated]

Western blot shows lysates of human brain (motor cortex) tissue. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24086) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for NCAM-1/CD56 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human peripheral blood was stained with either (A) Rabbit Anti-Human NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB24086) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by Anti-Rabbit IgG APC-conjugated secondary antibody (Catalog # F0111) and Mouse Anti-Human NKp46 PE-conjugated Monoclonal Antibody (Catalog # FAB1850P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 2524C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

NCAM-1/CD56 Antibody (2524C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line, NS0-derived human NCAM -1/CD56
  Leu20-Pro603 and Glu636-Asn741
  Accession # NP_001070150
• Specificity: Detects human NCAM-1/CD56 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NCAM-1/CD56 Antibody (2524C) [Unconjugated]

• CD56 / NCAM-1
• CD56 antigen
• CD56
• MSK39
• NCAM1
• N-CAM-1
• NCAM-1
• NCAMantigen recognized by monoclonal 5.1H11
• neural cell adhesion molecule 1
• neural cell adhesion molecule, NCAM

Background

Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca⁺⁺ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM‑140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM‑140 and NCAM-180 are type I transmembrane glycoproteins (4 - 6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7 ‑ 8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10 - 13).

1. Burg, M.A. et al. (1995) J. Neurosci. Res. 41:49.
2. Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124.
3. Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273.
4. Dickson, G. et al. (1987) Cell 50:1119.
5. Lanier, L.L. et al. (1991) J. Immunol. 146:4421.
6. Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71.
7. Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72.
8. Vawter, M.P. et al. (2001) Exp. Neurol. 172:29.
9. Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179.
10. Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143.
11. Doherty, P. et al. (1995) J. Neurobiol. 26:437.
12. Eckardt, M. et al. (2000) J. Neurosci. 20:5234.
13. Muller, D. et al. (1996) Neuron 17:413.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • NP_001070150()