NAT10 Antibody

Immunohistochemistry-Paraffin: NAT10 Antibody [NBP1-06055] - (3.8ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NAT10 Antibody Summary

• Immunogen: Peptide with sequence C-KSDKKRKLEAKQ corresponding to internal region (near C-Terminus) according to NP_078938.1.
• Epitope: C-KSDKKRKLEAKQ
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NAT10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments gave an approx. 26 kDa band in human brain (cerebellum, frontal cortex and hippocampus) lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116 kDa band according to NP_078938.1. The 26 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human colon shows nuclear staining in absorptive cells.
• Control:

  NAT10 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NAT10 Antibody

• ALP
• DKFZp434C116
• EC 2.3.1.-
• FLJ10774
• FLJ12179
• FLJ23850
• hALP
• KIAA1709
• N-acetyltransferase 10 (GCN5-related)
• N-acetyltransferase 10
• N-acetyltransferase-like protein
• NAT10
• NET43

Background

NAT10 has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, a

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NAT10 Antibody (NBP1-06055)(1)

Publication using NBP1-06055

• Yin J, Ma Z, Selliah N et al. Effective gene suppression using small interfering RNA in hard-to-transfect human T cells. J Immunol Methods 2006-05-30 [PMID: 16603179]

Bioinformatics

• Gene Symbol: NAT10
• Entrez:
  • 55226(Human)
  • 98956(Mouse)
• Uniprot:
  • NP_078938.1(Human)