NARF Antibody

Images

 
Western Blot: NARF Antibody [NBP2-19475] - Sample (30 ug of whole cell lysate) A: A549 B: MCF-7 7. 5% SDS PAGE gel, NARF antibody at 1:1000.
Immunocytochemistry/ Immunofluorescence: NARF Antibody [NBP2-19475] - IF analysis of paraformaldehyde-fixed HeLa cells, using NARF antibody at 1:500.
Immunohistochemistry-Paraffin: NARF Antibody [NBP2-19475] - Paraffin-embedded mouse forebrain section. NARF antibody at 1:250.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NARF Antibody Summary

Immunogen
NARF antibody is made to a recombinant protein encompassing a sequence within the center region of human NARF. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NARF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NARF Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP2-19475 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for NARF Antibody

  • FLJ10067
  • IOP2DKFZp434G0420
  • Iron-only hydrogenase-like protein 2
  • nuclear prelamin A recognition factor
  • prenyl-dependent prelamin A binding protein

Background

Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Review for NARF Antibody (NBP2-19475) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-19475:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot NARF NBP2-19475
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reviewed by:
Yongkang Yang
WB Human 08/08/2019
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell line A2780, MCF7
SpeciesHuman
Lot40807

Comments

CommentsWestern Blot: 1:1000

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Recent Reviews

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2
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1
0

Yongkang Yang
08/08/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol NARF
Uniprot