Recombinant B. subtilis NAPRT1 Protein

• Reactivity: Ba
• Applications: Enzyme Activity

Recombinant B. subtilis NAPRT1 Protein Summary

• Description: Full-length NAPRT1 (Bacillus subtilis).
  
  Source: E. coli

  Uniprot ID: O32090

• Details of Functionality: ~0.8 U/mg protein One unit is defined as the amount of enzyme that synthesizes 1umol nicotinic acid mononucleotide (NaMN) per min
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NAPRT
• Purity: >98%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM TRIS-HCl, pH 7.8, 50mM NaCl
• Preservative: No Preservative
• Purity: >98%, by SDS-PAGE

Alternate Names for Recombinant B. subtilis NAPRT1 Protein

• EC 2.4.2.11
• FHA-HIT-interacting protein
• FHIP
• NAPRTase
• nicotinate phosphoribosyltransferase domain containing 1
• Nicotinate phosphoribosyltransferase domain-containing protein 1
• nicotinate phosphoribosyltransferase
• nicotinic acid phosphoribosyltransferase
• PP3856

Background

Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NAPRT
• Uniprot:
  • O32090(B. subtilis)