NAPE-PLD Overexpression Lysate

Western Blot: NAPE-PLD Overexpression Lysate (Adult Normal) [NBL1-13476] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NAPE-PLD.

• Reactivity: Hu
• Applications: WB

NAPE-PLD Overexpression Lysate Summary

• Description: Transient overexpression lysate of N-acyl phosphatidylethanolamine phospholipase D (NAPEPLD), transcript variant 2 The lysate was created in HEK293T cells, using Plasmid ID RC209877 and based on accession number NM_198990. The protein contains a C-MYC/DDK Tag.
• Gene: NAPEPLD

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 45.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NAPE-PLD Overexpression Lysate

• C7orf18
• DKFZp781D1098
• EC 3.1.4
• EC 3.1.4.-
• N-acyl phosphatidylethanolamine phospholipase DNAPE-PLDFMP30
• N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D
• NAPE-hydrolyzing phospholipase D

Background

NAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]).[supplied by OMIM]. Transcript Variant: This variant (2) differs in the 3' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: NAPEPLD