NAPE-PLD Antibody

Western Blot: NAPE-PLD Antibody [NB110-80070] - Detection of NAPE-PLD antibody in DU145 cell extracts.

Western Blot: NAPE-PLD Antibody [NB110-80070] - Expression and activity of fatty acid amide hydrolase (FAAH) and NAPE-PLD in DRGs and spinal cord after nerve injury. QRT-PCR and Western Blot analysis of NAPE-PLD mRNA and protein expression in DRGs 7 days after SNI and treatment with R-flurbiprofen 4.5 mg/kg twice daily or vehicle. For QRT-PCR and Western Blot analysis pooled samples of each 3 mice were used and two independent experiments were performed. Mice were treated with 4.5 mg/kg R-flurbiprofen or vehicle twice daily (representative images; n = 6 per group). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0010628) licensed under a CC-BY license.

• Reactivity: Hu, Mu, Rt, Fu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NAPE-PLD Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of mouse NAPE-PLD (within residues 1-100). [Swiss-Prot# Q8BH82]
• Localization: Membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAPEPLD
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
  • Western Blot 1:2500-1:5000
• Application Notes: In Western blot, a band is seen at ~50 kDa. In ICC, this antibody has been tested in HT22 mouse neuronal cell line. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Fungi reactivity reported in scientific literature (PMID: 25433633)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for NAPE-PLD Antibody

• C7orf18
• DKFZp781D1098
• EC 3.1.4
• EC 3.1.4.-
• EC 3.1.4.54
• N-acyl phosphatidylethanolamine phospholipase DNAPE-PLDFMP30
• N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D
• NAPE-hydrolyzing phospholipase D
• NAPEPLD
• NAPE-PLD

Background

N-Acylphosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a membrane-bound enzyme which releases the endocannabinoid anandamide and other bioactive N-acylethanolamines from their corresponding NAPEs in animal tissues. NAPE-PLD has been shown to be expressed by specific populations of neurons in the brain and targeted to axonal processes. Recent studies have suggested that NAEs generated by NAPE-PLD in axons may act as anterograde synaptic signaling molecules that regulate the activity of postsynaptic neurons. NAPE-PLD has also been shown to catalyze the hydrolysis of NAPE to generate OEA (oleoylethanolamide), a natural lipid amide that inhibits food intake in free-feeding rodents by prolonging latency to feed and postmeal interval.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NAPE-PLD Antibody (NB110-80070)(9)

Publications using NB110-80070

• Piggott V, Lloyd S, Matchynski J et al. Traumatic Stress, Chronic Ethanol Exposure, or the Combination, Alter Cannabinoid System Components in Reward and Limbic Regions of the Mouse Brain Molecules 2021-04-30 [PMID: 33917316]
• Mai P, Yang L, Tian L et al. Endocannabinoid System Contributes to Liver Injury and Inflammation by Activation of Bone Marrow-Derived Monocytes/Macrophages in a CB1-Dependent Manner. J. Immunol. 2015-10-01 [PMID: 26320250] (WB, Mouse)
• Pacioni G, Rapino C, Zarivi O et al. Truffles contain endocannabinoid metabolic enzymes and anandamide. Phytochemistry. 2014-11-26 [PMID: 25433633] (WB, Mouse, Fungi)
  
  Details:
  NAPE-PLD antibody used for WB application in experiments involving the evaluation of endocannabinoid system (ECS) in black truffle Tuber melanosporum. 15 ug/lane of truffle homogenates prepared from powdered truffles (T. melanosporum) were analyzed on 10% SDS-PAGE with NAPE-PLD antibody used at 1:500 dilution. Lysate of mouse brain was included as a positive control and the antibody detected the target band around 46kDa (Fig. 1b).
• Cecyre B, Zabouri N, Huppe-Gourgues F et al. Roles of cannabinoid receptors type 1 and 2 on the retinal function of adult mice. Invest Ophthalmol Vis Sci 2013-12-11 [PMID: 24255040]
• Bari M, Battista N, Valenza M et al. In vitro and in vivo models of Huntington's disease show alterations in the endocannabinoid system. FEBS J 2013-05-09 [PMID: 23659592] (WB, ICC/IF, Rat)
• Lewis SE, Rapino C, Di Tommaso M et al. Differences in the endocannabinoid system of sperm from fertile and infertile men PLoS One 2012-01-01 [PMID: 23082196] (WB, Human)
• Battista N, Di Sabatino A, Di Tommaso M et al. Abnormal anandamide metabolism in celiac disease. The Journal of Nutritional Biochemistry. 2011-12-29 [PMID: 22209002]
• Zabouri N, Bouchard, J-F, Casanova C et al. Cannabinoid receptor type 1 expression during postnatal development of the rat retina. The Journal of Comparative Neurology [PMID: 21452196] (WB, Rat)
• Bishay P, Schmidt H et al. R-flurbiprofen reduces neuropathic pain in rodents by restoring endogenous cannabinoids. PLoS One 2010-05-26 [PMID: 20498712] (WB, Rat)

Bioinformatics

• Gene Symbol: NAPEPLD
• Entrez:
  • 222236(Human)
  • 242864(Mouse)
  • 296757(Rat)
• Uniprot:
  • Q8BH82(Mouse)