NAPE-PLD Antibody

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Western Blot: NAPE-PLD Antibody [NB110-80070] - Detection of NAPE-PLD antibody in DU145 cell extracts.

Product Details

Summary
Reactivity Hu, Mu, Rt, FuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NAPE-PLD Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of mouse NAPE-PLD (within residues 1-100). [Swiss-Prot# Q8BH82]
Localization
Membrane.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NAPEPLD
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2500-1:5000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
Application Notes
This NAPE-PLD antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~50 kDa. In ICC, this antibody has been tested in HT22 mouse neuronal cell line.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Brain Lysate (NB820-59657)
Publications
Read Publications using
NB110-80070 in the following applications:

Reactivity Notes

Human, Mouse and Rat. Fungi reactivity reported in scientific literature (PMID: 25433633)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for NAPE-PLD Antibody

  • C7orf18
  • DKFZp781D1098
  • EC 3.1.4
  • EC 3.1.4.-
  • N-acyl phosphatidylethanolamine phospholipase DNAPE-PLDFMP30
  • N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D
  • NAPE-hydrolyzing phospholipase D

Background

N-Acylphosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a membrane-bound enzyme which releases the endocannabinoid anandamide and other bioactive N-acylethanolamines from their corresponding NAPEs in animal tissues. NAPE-PLD has been shown to be expressed by specific populations of neurons in the brain and targeted to axonal processes. Recent studies have suggested that NAEs generated by NAPE-PLD in axons may act as anterograde synaptic signaling molecules that regulate the activity of postsynaptic neurons. NAPE-PLD has also been shown to catalyze the hydrolysis of NAPE to generate OEA (oleoylethanolamide), a natural lipid amide that inhibits food intake in free-feeding rodents by prolonging latency to feed and postmeal interval.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NAPE-PLD Antibody (NB110-80070)(7)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Fungi.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(5)
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Human
(1)
Mouse
(2)
Rat
(2)
Fungi
(1)
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Showing Publications 1 - 7 of 7.
Publications using NB110-80070 Applications Species
Mai P, Yang L, Tian L et al. Endocannabinoid System Contributes to Liver Injury and Inflammation by Activation of Bone Marrow-Derived Monocytes/Macrophages in a CB1-Dependent Manner. J. Immunol. 2015 Oct 01 [PMID: 26320250] (WB, Mouse) WB Mouse
Pacioni G, Rapino C, Zarivi O et al. Truffles contain endocannabinoid metabolic enzymes and anandamide. Phytochemistry. 2014 Nov 26 [PMID: 25433633] (WB, Fungi, Mouse)

Details:
NAPE-PLD antibody used for WB application in experiments involving the evaluation of endocannabinoid system (ECS) in black truffle Tuber melanosporum. 15 ug/lane of truffle homogenates prepared from powdered truffles (T. melanosporum) were analyzed on 10% SDS-PAGE with NAPE-PLD antibody used at 1:500 dilution. Lysate of mouse brain was included as a positive control and the antibody detected the target band around 46kDa (Fig. 1b).
WB Fungi, Mouse
Cecyre B, Zabouri N, Huppe-Gourgues F et al. Roles of cannabinoid receptors type 1 and 2 on the retinal function of adult mice. Invest Ophthalmol Vis Sci 2013 Dec 11 [PMID: 24255040]
Bari M, Battista N, Valenza M et al. In vitro and in vivo models of Huntington's disease show alterations in the endocannabinoid system. FEBS J 2013 May 9 [PMID: 23659592] (WB, ICC/IF, Rat) WB, ICC/IF Rat
Lewis SE, Rapino C, Di Tommaso M et al. Differences in the endocannabinoid system of sperm from fertile and infertile men PLoS One 2012 [PMID: 23082196] (WB, Human) WB Human
Battista N, Di Sabatino A, Di Tommaso M et al. Abnormal anandamide metabolism in celiac disease. The Journal of Nutritional Biochemistry. 2011 Dec 29. [PMID: 22209002]
Zabouri N, Bouchard, J-F, Casanova C et al. Cannabinoid receptor type 1 expression during postnatal development of the rat retina. The Journal of Comparative Neurology [PMID: 21452196] (WB, Rat) WB Rat

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NAPEPLD
Entrez
Uniprot