N-PAC Antibody - Azide and BSA Free

Western Blot: N-PAC Antibody [NBP2-99354] - Anti-N-PAC rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa

Immunocytochemistry/Immunofluorescence: N-PAC Antibody [NBP2-99354] - Immunofluorescence staining of N-PAC in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99354 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

N-PAC Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human N-PAC.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLYR1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for N-PAC Antibody - Azide and BSA Free

• BM045
• Cytokine-like nuclear factor N-PAC
• glyoxylate reductase 1 homolog (Arabidopsis)
• Glyoxylate reductase 1 homolog
• HIBDLnuclear protein 60 kDa
• NP603-hydroxyisobutyrate dehydrogenase-like protein
• N-PAC
• nuclear protein 60kDa
• Nuclear protein NP60
• Nuclear protein of 60 kDa
• putative oxidoreductase GLYR1

Background

N-PAC may have oxidoreductase activity. Regulates p38 MAP kinase activity by mediating stress activation ofp38alpha/MAPK14 and specifically regulating MAPK14 signaling. Indirectly promotes phosphorylation of MAPK14 andactivation of ATF2. The phosphorylation of

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLYR1