n-Myc Antibody (NMYC-1) [Alexa Fluor™ Plus 647]

• Reactivity: Hu, Mu
• Applications: WB, ChIP, Flow, GS, ICC/IF, IP, ChIP, CyTOF-ready
• Clone: NMYC-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

n-Myc Antibody (NMYC-1) [Alexa Fluor™ Plus 647] Summary

• Immunogen: A synthetic peptide (SPYVESEDAPPQKC) conjugated to KLH, corresponding to amino acids 327-339 of human n-Myc. [UniProt# P04198]
• Specificity: This antibody does not react with c-Myc or L-Myc.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYCN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation
  • CyTOF-ready
  • Flow Cytometry
  • Gel Super Shift Assays
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for n-Myc Antibody (NMYC-1) [Alexa Fluor™ Plus 647]

• BHLHE37
• bHLHe37N-myc proto-oncogene protein
• Class E basic helix-loop-helix protein 37
• MODED
• neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene
• N-myc
• NMYCneuroblastoma MYC oncogene
• ODED
• oncogene NMYC
• pp65/67
• v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived
• v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)

Background

n-Myc is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYCN