Myosin Light Chain 2 Antibody (7C9)

Western Blot: Myosin Light Chain 2 Antibody (7C9) [NBP1-28871] - Western blot analysis using MYL3 (1) and MYL2 (2) mouse mAb against rat fetal heart tissue lysate.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Single-Cell Western
• Clone: 7C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Myosin Light Chain 2 Antibody (7C9) Summary

• Description: Novus Biologicals Mouse Myosin Light Chain 2 Antibody (7C9) (NBP1-28871) is a monoclonal antibody validated for use in WB and ELISA. Anti-Myosin Light Chain 2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of Myosin Light Chain 2 expressed in E. Coli.
• Specificity: Specific for MYL2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYL2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Single Cell Western 1:10
  • Western Blot 1:500 - 1:2000
• Application Notes: Single Cell Western reported by an internal validation at a 1:10 dilution
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Myosin Light Chain 2 Antibody (7C9)

• cardiac ventricular myosin light chain 2
• CMH10myosin light chain 2
• DKFZp779C0562
• MLC2
• MLC-2
• MLC-2v
• myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• myosin, light chain 2, regulatory, cardiac, slow
• myosin, light polypeptide 2, regulatory, cardiac, slow
• regulatory light chain of myosin
• RLC of myosin
• slow cardiac myosin regulatory light chain 2

Background

MYL2 (myosin, light chain 2, regulatory, cardiac, slow), also known as MLC-2, MLC2v. Entrez protein NP_000423. MYL2 associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. It is an hexamer of two heavy chains and four light chains that is predominantly expressed in adult cardiac ventricle muscle. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myosin Light Chain 2 Antibody (NBP1-28871)(1)

Publication using NBP1-28871

• Aguanno, D, Coquant, G Et al. The intestinal quorum sensing 3-oxo-C12:2 Acyl homoserine lactone limits cytokine-induced tight junction disruption. Tissue Barriers 2020-10-01 [PMID: 33100129] (WB, Human)

Bioinformatics

• Gene Symbol: MYL2