Myosin Light Chain 2 Antibody (3B2) [DyLight 488]

• Reactivity: Hu, Mu, Xp
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 3B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 488

Myosin Light Chain 2 Antibody (3B2) [DyLight 488] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Recombinant human Myosin Light Chain 2 (1-166aa) purified from E. coli (NP_000423).
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYL2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP1-30249. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Xenopus reactivity reported in scientific literature (PMID: 23727888). Mouse reactivity reported in scientific literature (PMID: 28430962).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Myosin Light Chain 2 Antibody (3B2) [DyLight 488]

• cardiac ventricular myosin light chain 2
• CMH10myosin light chain 2
• DKFZp779C0562
• MLC2
• MLC-2
• MLC-2v
• myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• myosin, light chain 2, regulatory, cardiac, slow
• myosin, light polypeptide 2, regulatory, cardiac, slow
• regulatory light chain of myosin
• RLC of myosin
• slow cardiac myosin regulatory light chain 2

Background

MYL2 (myosin, light chain 2, regulatory, cardiac, slow), also known as MLC-2, MLC2v. Entrez protein NP_000423. MYL2 associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. It is an hexamer of two heavy chains and four light chains that is predominantly expressed in adult cardiac ventricle muscle. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myosin Light Chain 2 Antibody (NBP1-30249G)(1)

Publication using NBP1-30249G

• Cui C, Wang J, Qian D et al. Binary Colloidal Crystals Drive Spheroid Formation and Accelerate Maturation of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes ACS Appl Mater Interfaces. 2019-01-30 [PMID: 30614683] (FLOW, Human)
  
  Details:
  Citation using the DyLight 488 form of this antibody.

Bioinformatics

• Gene Symbol: MYL2
• Entrez:
  • 4633(Human)
  • 363925(Rat)
• Uniprot:
  • P10916(Human)