Myosin heavy chain 2 Antibody (4G9) - BSA Free

Western Blot: Myosin heavy chain 2 Antibody (4G9) [NBP3-43001] - human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with (Myosin heavy chain 2 Antibody) at dilution of 1:4000

Immunohistochemistry: Myosin heavy chain 2 Antibody (4G9) [NBP3-43001] - Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using (Myosin heavy chain 2 Antibody) at dilution of 1:100

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 4G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Myosin heavy chain 2 Antibody (4G9) - BSA Free Summary

• Immunogen: myosin, heavy chain 2, skeletal muscle, adult - (Uniprot# Q9UKX2)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYH2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20 - 1:200
  • Western Blot 1:5000 - 1:10000
• Theoretical MW: 200 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Myosin heavy chain 2 Antibody (4G9) - BSA Free

• fast 2a myosin heavy chain
• MYH2
• MYH2A
• MYHas8
• MyHC-2a
• MyHC-IIa
• MYHSA2
• Myosin heavy chain 2
• myosin heavy chain 2a
• myosin heavy chain IIa
• myosin, heavy chain 2, skeletal muscle, adult
• myosin, heavy polypeptide 2, skeletal muscle, adult
• myosin-2
• skeletal muscle, adult 2
• type IIA myosin heavy chain

Background

Myosin II is the so called "classical" myosin, being the first type of myosin to be discovered. Myosin II forms bipolar filaments that interact with actin filaments to produce contraction. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. The myosin II molecule is composed of two heavy chains and two sets of light chains. The light chains are the Essential Light Chains (ELC) and Regulatory Light Chains (RLC). Overall activity and polymerization can be modulated by phosphorylation on the light chains and the C terminus of the heavy chains respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYH2