Myelin PLP Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Myelin PLP Antibody [NB100-1608] - Immunohistochemistry photomicrograph of PLP immunoreactivity (Green; 1:1000 dilution) in a cryostat section of an embryonic (e18) mouse brain. ...read more
Immunohistochemistry-Frozen: Myelin PLP Antibody [NB100-1608] - Human brain and spinal cord stained with Myelin PLP antibody (green) at 1:3000 dilution.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

Myelin PLP Antibody Summary

Immunogen
Chickens were immunized with a synthetic peptide/keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of Myelin PLP, but are shared between the human (NP_000524, PLP1 isoform 1 and PLP1 isoform 2 (NP_955772)] and mouse (P60202).
Marker
Myelin Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
PLP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:2000 - 1:5000
  • Immunohistochemistry-Frozen
  • Western Blot 1:2000 - 1:5000
Application Notes
Quality assurance analysis was performed using immunohistochemistry (1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500) as the secondary reagent. Western Blot reactivity reported in scientific literature (PMID: 24057669).
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-1608 in the following applications:

Publications
Read Publication using
NB100-1608 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Sodium PBS (0.9% isotonic, w/v, pH 7.2)
Preservative
0.02% Sodium Azide
Concentration
0.3 mg/ml
Purity
Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Purification Notes
After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentration of the eluate adjusted to 100 ug/ml, and the preparation was filter sterilized.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Alternate Names for Myelin PLP Antibody

  • DM-20
  • HLD1
  • Lipophilin
  • major myelin proteolipid protein
  • MMPL
  • Myelin PLP
  • myelin proteolipid protein
  • PLP
  • PLP/DM20
  • PLP1
  • PMD
  • proteolipid protein 1
  • spastic paraplegia 2, uncomplicated
  • SPG2

Background

Human PLP (also known as lipophilin) is a 30 kDa protein (277 amino acids) found in myelin of the CNS and PNS. Expressed by oligodendrocytes and Schwann cells, PLP stabilizes myelin by preventing lipid bilayer fusion, and aids in compaction. Different mutations of the human PLP gene product result in two neurological disorders - Pelizaeus-Merzbacher disease and Spastic Paraplegia type 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myelin PLP Antibody (NB100-1608)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Mouse
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Review for Myelin PLP Antibody (NB100-1608) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: homo sapiens and Human.

Reviews using NB100-1608:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
homo sapiens and Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Myelin PLP NB100-1608
Enlarge
5
reviewed by:
Verified Customer
ICC homo sapiens and Human 10/22/2021
View

Summary

ApplicationImmunocytochemistry
Sample Tested brain and spinal cord
Specieshomo sapiens and Human
Lotplp0305

Product General Protocols

View specific protocols for Myelin PLP Antibody (NB100-1608): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Myelin PLP Antibody (NB100-1608) (0)

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Secondary Antibodies

 

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Recent Reviews

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1
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Verified Customer
10/22/2021
Application: ICC
Species: homo sapiens and Human

Bioinformatics

Gene Symbol PLP1
Uniprot