Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (1D1)

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (1D1) [H00005213-M04] - Analysis of PFKM expression in K-562 (Cat # L009V1).

Sandwich ELISA: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (1D1) [H00005213-M04] - Detection limit for recombinant GST tagged PFKM is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, S-ELISA
• Clone: 1D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (1D1) Summary

• Immunogen: PFKM (NP_000280, 681 a.a. ~ 780 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV
• Specificity: PFKM - phosphofructokinase, muscle (1D1)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PFKM
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (1D1)

• 6-phosphofructokinase, muscle type
• EC 2.7.1,6-phosphofructo-1-kinase
• EC 2.7.1.11
• GSD7
• MGC8699
• Muscle Phosphofructokinase
• PFK1
• PFK-1
• PFKA
• PFK-A
• PFKM
• PFKX
• Phosphofructo-1-kinase isozyme A
• Phosphofructokinase 1
• phosphofructokinase, muscle
• phosphofructokinase, polypeptide X
• Phosphofructokinase-1
• Phosphofructokinase-M
• Phosphohexokinase

Background

The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL; MIM 171860), and platelet (PFKP; MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721]; Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PFKM
• Entrez:
  • 5213(Human)
• Uniprot:
  • NP_000280(Human)