Synthetic peptides corresponding to TRIM54(tripartite motif-containing 54) The peptide sequence was selected from the N terminal of TRIM54.
Peptide sequence NFTVGFKPLLGDAHSMDNLEKQLICPICLEMFSKPVVILPCQHNLCRKCA.
Immunogen affinity purified
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The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Alternate Names for MURF3 Antibody
MURFtripartite motif-containing protein 54
Muscle-specific RING finger protein 3
Muscle-specific RING finger protein
RING finger protein 30muscle-specific RING-finger protein 3
tripartite motif containing 54
tripartite motif-containing 54
TRIM54 contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Subcellular Fraction: RIPA diluted in 1% proteinase inhibitor of PBS
Concentration: 1 µg/µl
Preparation: 1. Perform a protein quantification assay (Bio-Red). Determine the protein concentration for each cell lysate. 2. Determine how much protein to load and add an equal volume 2X Laemmli sample buffer. 3. Boil each cell lysate in sample buffer at 100°C for 5 min.
Controls: no positive control
PAGE Gel: 10%
PAGE Conditions: 60 volt for 1h, then 95 volt for 1-2h
Membrane: PVDF 0.45µm
Transfer Conditions: 35 volt, overnight
Blocking Solution/ Duration: 5% milk in PBST for 1h at room temperature
Primary Antibody Storage Conditions: -20C
Reconstitution & Aliquot information: no
Primary Antibody Diluent and Dilution: 1:500 in PBST contained 5% BSA
Primary Antibody Incubation Time and Temperature: 4C overnight
Wash Solution Composition, Repetitions & Times: PBST, three washes, 10 min each
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