MUC6 Antibody (CLH5) - IHC-Prediluted

Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) - IHC-Prediluted [NBP2-48339] - Formalin-fixed, paraffin-embedded human normal stomach. Strong cytoplasmic of glandular cells using MUC6 Antibody (CLH5). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) - IHC-Prediluted [NBP2-48339] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC6 Antibody (CLH5). Courtesy of Dr. Leonor David, IPATIMUP and Medical Faculty, University of Porto, Portugal

• Reactivity: Hu
• Applications: IHC
• Clone: CLH5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

MUC6 Antibody (CLH5) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: A synthetic peptide of the Gastric MUC6 tandem repeat sequence (Uniprot: Q6W4X9 )
• Localization: Cytoplasmic & Secreted
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUC6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Hu-chromosome location: 11p15.5 Immunohistochemistry-Paraffin 2 - 4 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 252 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for MUC6 Antibody (CLH5) - IHC-Prediluted

• gastric mucin
• Gastric mucin-6
• MUC-6
• mucin 6, gastric
• mucin 6, oligomeric mucus/gel-forming
• mucin-6

Background

Mucin is a high M.W. (>1,000 kDa) glycoprotein, expressed by mucus cells of the gastric epithelium and by goblet cells of the fetal, precancerous and cancerous colon, but not by those of the normal colon. It also appears in other epithelial tissues, which are embryologically derived from the foregut (epigastric and bronchial epithelium) and in M ller ducts (mucus cells of the endocervix and urethral epithelium near the prostatic utriculus).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MUC6 Antibody (NBP2-48339)(1)

Publication using NBP2-48339

• Aoki Y, Mizuma M, Hata T et al. Intraductal papillary neoplasms of the bile duct are consisted of two distinct types specifically associated with clinicopathological features and molecular phenotypes J Pathol. [PMID: 32100878] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: MUC6