MUC5B Antibody (19.4E) - BSA Free

• Reactivity: Hu, Pm, Mu(-)
• Applications: WB, ELISA, IHC
• Clone: 19.4E
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MUC5B Antibody (19.4E) - BSA Free Summary

• Description: Relevant Entrez Gene Numbers: NM_002458.1
• Immunogen: Un-glycosylated, serine- and threonine-free synthetic peptide from the N-terminus of human MUC5B.
• Specificity: Reacts with Mucin 5B (MUC5B). Does not react with human Mucin 5A or 5C.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUC5B
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Western Blot; Bands in the high molecular weight range of 200kDa or above are expected for highly glycosylated proteins. Immunohistchemistry: Paraffin sections. Deglycosylating the tissue is critical to expose epitope. There are a number of deglycosylation protocols available and a discussion of these related to Mucin can be found in Cao, Y. et al. (1997)(see references).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Liquid in 0.02M Phosphate buffer, 0.25 M NaCl.
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Notes

Relevant Entrez Gene Numbers: NM_002458.1

Alternate Names for MUC5B Antibody (19.4E) - BSA Free

• high molecular weight salivary mucin MG1
• mucin 5, subtype B, tracheobronchial
• mucin 5B, oligomeric mucus/gel-forming
• sublingual gland mucin
• tracheobronchial

Background

Mucins are a family of highly glycosylated proteins that are present on the surface of, and are secreted by, many glandular epithelial cells. Mucins provide a barrier between the luminal membranes of cells in the epithelium and their environment. For instance, in the digestive tract, the mucin barrier protects the epithelial cells from the action of proteolytic enzymes. These high molecular weight glycoproteins consist of a nonglobular protein backbone (apomucin) and many O-linked glycans. Salivary Mucin 5B proteins are some of the largest polypeptides, with molecular weights ranging from 14 - 40 x 10E3 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MUC5B
• Uniprot:
  • Q9HC84(Human)