MUC4 Antibody (781631) [Allophycocyanin]

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human MUC-4 APC-conjugated Monoclonal Antibody (Catalog # FAB8195A, filled histogram) or isotype control antibody (IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 781631
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

MUC4 Antibody (781631) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant human MUC-4
  Pro1072-Ser1317
  Accession # Q99102
• Specificity: Detects human MUC-4 in ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUC4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MUC4 Antibody (781631) [Allophycocyanin]

• Ascites sialoglycoprotein
• ASGP
• HSA276359
• MUC4
• MUC-4
• mucin 4, cell surface associated
• mucin 4, tracheobronchial
• mucin-4
• Pancreatic adenocarcinoma mucin
• Testis mucin
• Tracheobronchial mucin

Background

MUC-4 (Mucin-4), also called ASGP (ascites sialoglycoprotein) is a highly glycosylated type I transmembrane glycoprotein that may be up to 950 kDa in its full-length, fully glycosylated form. Human MUC-4 cDNA encodes 2169 amino acids (aa) with a 28 aa signal sequence and a cleavage site that creates a 1416 aa soluble, extracellular alpha chain and a 725 aa single-pass transmembrane beta chain. Between aa 1072-1317 within the alpha chain, human MUC-4 shares 69% aa sequence identity with mouse and rat MUC-4. At least 14 soluble or transmembrane splice variants of 1102-2117 aa have been described, 5 of which contain the full sequence used as an immunogen. MUC-4 can serve as a ligand for the oncogenic receptor ErbB2 and a modulator of its phosphorylation and signaling. MUC-4 is frequently aberrantly expressed in epithelial tumors and can promote tumor growth and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MUC4
• Uniprot:
  • Q99102()