MUC1 Antibody (GP1.4) [DyLight 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
GP1.4
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Order Details

MUC1 Antibody (GP1.4) [DyLight 488] Summary

Immunogen
Human milk fat globule membrane was used as the immunogen for the EMA antibody.
Localization
Cytoplasmic & cell surface
Marker
Epithelial Marker
Specificity
In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This MAb reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MUC1 Antibody (GP1.4) [DyLight 488]

  • Breast carcinoma-associated antigen DF3
  • Carcinoma-associated mucin
  • CD227 antigen
  • CD227
  • DF3 antigen
  • EMA
  • Episialin
  • H23 antigen
  • H23AG
  • KL-6
  • MAM6
  • MUC-1
  • MUC1/ZD
  • mucin 1, cell surface associated
  • mucin 1, transmembrane
  • Mucin-1
  • Peanut-reactive urinary mucin
  • PEM
  • PEMMUC-1/SEC
  • PEMT
  • Polymorphic epithelial mucin
  • PUMMUC-1/X
  • tumor associated epithelial mucin
  • Tumor-associated epithelial membrane antigen
  • Tumor-associated mucin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MUC1 Antibody (NBP2-33174G). (Showing 1 - 1 of 1 FAQ).

  1. I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
    • Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-33174AF350
Alexa Fluor 405 NBP2-33174AF405
Alexa Fluor 488 NBP2-33174AF488
Alexa Fluor 532 NBP2-33174AF532
Alexa Fluor 594 NBP2-33174AF594
Alexa Fluor 647 NBP2-33174AF647
Alexa Fluor 700 NBP2-33174AF700
Alexa Fluor 750 NBP2-33174AF750
Allophycocyanin NBP2-33174APC
Allophycocyanin/Cy7 NBP2-33174APCCY7
Biotin NBP2-33174B
DyLight 350 NBP2-33174UV
DyLight 405 NBP2-33174V
DyLight 488 NBP2-33174G
DyLight 550 NBP2-33174R
DyLight 594 NBP2-33174DL594
DyLight 650 NBP2-33174C
DyLight 680 NBP2-33174FR
DyLight 755 NBP2-33174IR
FITC NBP2-33174F
HRP NBP2-33174H
Janelia Fluor 549 NBP2-33174JF549
Janelia Fluor 646 NBP2-33174JF646
PE NBP2-33174PE
PE/Atto594 NBP2-33174PEATT594
PE/Cy5.5 NBP2-33174PECY55
PE/Cy7 NBP2-33174PECY7
PerCP NBP2-33174PCP

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Contact Information

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Product PDFs

Calculators

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Bioinformatics

Gene Symbol MUC1