MUC1 Antibody (GP1.4) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: GP1.4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

MUC1 Antibody (GP1.4) [Alexa Fluor® 350] Summary

• Immunogen: Human milk fat globule membrane was used as the immunogen for the EMA antibody.
• Localization: Cytoplasmic & cell surface
• Marker: Epithelial Marker
• Specificity: In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This MAb reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MUC1 Antibody (GP1.4) [Alexa Fluor® 350]

• Breast carcinoma-associated antigen DF3
• Carcinoma-associated mucin
• CD227 antigen
• CD227
• DF3 antigen
• EMA
• Episialin
• H23 antigen
• H23AG
• KL-6
• MAM6
• MUC-1
• MUC1/ZD
• mucin 1, cell surface associated
• mucin 1, transmembrane
• Mucin-1
• Peanut-reactive urinary mucin
• PEM
• PEMMUC-1/SEC
• PEMT
• Polymorphic epithelial mucin
• PUMMUC-1/X
• tumor associated epithelial mucin
• Tumor-associated epithelial membrane antigen
• Tumor-associated mucin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MUC1 Antibody (NBP2-33174AF350). (Showing 1 - 1 of 1 FAQ).

1. I would like to perform a sandwich assay for the determination of MUC1 protein. May you suggest me some antibodies that can be used together? In particular, if possible, I should need antibodies product in mouse or rabbit.
   • Unfortunately we are not aware of a pair of antibodies that have been used in sandwich ELISA in particular. If you are interested in trying two you may find our Innovators Reward Program to be helpful.

Bioinformatics

• Gene Symbol: MUC1