Recombinant Human MTHFS His Protein

SDS-Page: Recombinant Human MTHFS Protein [NBP1-51046] - 15 % SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human MTHFS His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-203 of Human MTHFS
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAAAVSSAK RSLRGELKQR LRAMSAEERL RQSRVLSQKV IAHSEYQKSK RISIFLSMQD EIETEEIIKD IFQRGKICFI PRYRFQSNHM DMVRIESPEE ISLLPKTSWN IPQPGEGDVR EEALSTGGLD LIFMPGLGFD KHGNRLGRGK GYYDAYLKRC LQHQEVKPYT LALAFKEQIC LQVPVNENDM KVDEVLYEDS STA

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MTHFS
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 25.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 5mM DTT, 30% glycerol, 0.2 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human MTHFS His Protein

• 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolatecyclo-ligase)
• 5-formyltetrahydrofolate cyclo-ligase
• EC 6.3.3.25,10-methenyl-tetrahydrofolate synthetase
• FLJ30410
• HsT19268
• Methenyl-THF synthetase

Background

MTHFS, also known as 5-formyltetrahydrofolate cyclo-ligase, is a cytosolic protein involved in the formate metabolic process. MTHFS, with a magnesium cofactor, catalyzes the ATP-dependent reaction that reduces 5-formyltetrahydrofolate (5-MTHF) to 5,10-methenyltetrahydrofolate(MTHF). MTHF is the substrate used by MTHFR (methylenetetrahydrofolate reductase) to generate 5-MTHF. MTHF is also a coenzyme used in thymidine biosynthesis by thymidylate synthase (FAD). Recombinant human MTHFS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MTHFS