MTHFD1L Antibody

Western Blot: MTHFD1L Antibody [NBP1-78279] - A. NIH3T3, B. Mouse Brain and C. Rat Brain lysates (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: MTHFD1L Antibody [NBP1-78279] - Human Tonsil tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: MTHFD1L Antibody [NBP1-78279] - Human Cer (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MTHFD1L Antibody Summary

• Immunogen: Peptide with sequence C-DTNDRFLRKITIGQ corresponding to internal region according to NP_056255.2.
• Epitope: aa535-538
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MTHFD1L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: WB: Approx. 110 kDa band observed in human brain (cerebellum) (calculated MW of 106 kDa band according to NP_056255.2). The same band is observed in lysates of NIH3T3, mouse brain and rat brain.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MTHFD1L Antibody

• 10-formyl-THF synthetase
• dJ292B18.2
• DKFZp586G1517
• EC 6.3.4.3
• FLJ21145
• formyltetrahydrofolate synthetase domain containing 1
• Formyltetrahydrofolate synthetase
• FTHFSDC1
• methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
• mitochondrial C1-tetrahydrofolate synthase
• mitochondrial C1-tetrahydrofolate synthetase
• monofunctional C1-tetrahydrofolate synthase, mitochondrial
• MTC1THFS

Background

MTHFD1L, also known as Monofunctional C1-tetrahydrofolate synthase, mitochondrial, consists of 2 a 978 amino acid isoform1 that is 106 kDa and a 275 amino acid isoform 2 that is 30 kDa; has mitochondrion subcellular location and highest expression found in placenta, thymus and brain; it is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion which is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine, and also may provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. Current research is being performed on this protein involvement in cleft lip/palate, neural tube defect, homocysteine, coronary heart disease, chronic myeloid leukemia, Down syndrome, colon adenocarcinoma, nephropathy, Alzheimer's disease, dementia, colorectal cancer, leukemia, and atherosclerosis. This protein plays role in One carbon pool by folate and Metabolic pathways where it interacts with CASP4, MAGED1, WRAP73, WDR8, MAP1LC3A, and plus more than 40 other proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MTHFD1L Antibody (NBP1-78279)(1)

Publication using NBP1-78279

• Parle-McDermott A, Pangilinan F, O'Brien KK et al. A common variant in MTHFD1L is associated with neural tube defects and mRNA splicing efficiency. Hum Mutat 2009-12-01 [PMID: 19777576]

Bioinformatics

• Gene Symbol: MTHFD1L
• Entrez:
  • 270685(Mouse)
  • 361472(Rat)